Plapp F W, Tripathi R K
Biochem Genet. 1978 Feb;16(1-2):1-11. doi: 10.1007/BF00484380.
Resistance to the organophosphate insecticide tetrachlorvinphos was examined in a house fly (Musca domestica L.) strain with an altered acetylcholinesterase (AChE) of decreased sensitivity to inhibition by the insecticide. Genetic tests showed that both resistance and the altered AChE were controlled by semi-dominant gene(s) on chromosome II. The gene for resistance was five crossover units from the mutant marker stubby wing (stw). A house fly strain was prepared in which resistance was introduced in to a susceptible stw strain by recombination. Biochemical assays revealed that the altered AChE was introduced along with resistance. Assays of the AChE of resistant and susceptible stw strains by two independent methods showed that the enzyme from resistant flies was 30 times more slowly inhibited by tetrachlorvinphos than the enzyme from susceptible flies.
在一种家蝇（Musca domestica L.）品系中检测了对有机磷酸酯类杀虫剂毒死蜱的抗性，该品系的乙酰胆碱酯酶（AChE）发生了改变，对该杀虫剂抑制作用的敏感性降低。遗传学测试表明，抗性和改变的AChE均由位于第二染色体上的半显性基因控制。抗性基因与突变标记短翅（stw）相距5个交换单位。制备了一个家蝇品系，通过重组将抗性导入到一个敏感的stw品系中。生化分析表明，改变的AChE与抗性一同被导入。通过两种独立方法对抗性和敏感stw品系的AChE进行测定，结果显示，抗性家蝇的酶被毒死蜱抑制的速度比敏感家蝇的酶慢30倍。
