Department of Animal Biosciences, University of Guelph, Guelph, Canada.
College of Animal Life Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Biol Trace Elem Res. 2022 Mar;200(3):1321-1330. doi: 10.1007/s12011-021-02747-6. Epub 2021 May 5.
This experiment was conducted to investigate the effect of the supplementation of hot-melt extrusion (HME) processed zinc sulfate (ZnSO) on the growth performance, antioxidative activity, pancreatic digestive enzyme, small intestinal morphology, nutrient digestibility, and Zn content in broilers. The chicks were allocated to three treatments, each of which had five replicates of 15 chicks per replicate. The broiler chickens were assigned to three dietary treatments: the control (without supplemental Zn), IN-Zn (ZnSO, 80 mg/kg), and HME-Zn (HME processed ZnSO as nano-Zn, 80 mg/kg). The broilers fed diets supplemented with 80 mg/kg of HME-Zn improved the BWG (P < 0.05) and FCR (P < 0.05) compared to the broilers fed the control and IN-Zn diets in phase 2. The Zn supplementation significantly enhanced the superoxide dismutase (SOD) activity in the serum (P < 0.05) and liver (P < 0.05), and HME-Zn supplementation significantly increased the SOD in the liver compared to the IN-Zn supplementation. Reduced malondialdehyde (MDA) concentration was seen with the Zn supplementation compared to the control (P < 0.05). The chickens fed diets supplemented with the HME-Zn had higher activity of amylase (P < 0.05) and trypsin (P < 0.05) than those of the chickens fed the control and IN-Zn diets. The villus height (VH) in the duodenum (P < 0.05) and jejunum (P < 0.05) increased with the ZnSO and HME-Zn supplementation compared to the control. The VH and crypt depth rate (VH:CD) in the jejunum improved with the HME-Zn compared to the control (P < 0.05). The HME-Zn significantly increased the apparent ileal digestible crude protein (CP) (P < 0.05) and energy corrected by nitrogen (AIDEn) (P < 0.05) compared to the control or IN-Zn. In phases 1 and 2, the HME-Zn significantly increased Zn concentration in the liver and tibia compared to control and IN-Zn (P < 0.05). The excretion of Zn was significantly decreased in the HME-Zn compared to the IN-Zn (P < 0.05). In conclusion, supplementation of 80 mg/kg of HME-Zn in diets improved the growth performance, antioxidative activity, pancreatic enzyme activity, intestinal villus height, and nutrient digestibility with the improved Zn bioavailability in broilers.
本实验旨在研究热熔挤出（HME）加工硫酸锌（ZnSO）对肉鸡生长性能、抗氧化活性、胰腺消化酶、小肠形态、养分消化率和锌含量的影响。将雏鸡分为 3 个处理组，每个处理组有 5 个重复，每个重复有 15 只雏鸡。肉鸡被分配到三种饮食处理：对照组（不补充锌）、IN-Zn（ZnSO，80mg/kg）和 HME-Zn（HME 处理的 ZnSO 为纳米 Zn，80mg/kg）。与对照组和 IN-Zn 组相比，饲喂 80mg/kg HME-Zn 的肉鸡在第 2 阶段提高了平均日增重（BWG）（P<0.05）和饲料转化率（FCR）（P<0.05）。锌补充显著提高了血清（P<0.05）和肝脏（P<0.05）中超氧化物歧化酶（SOD）的活性，与 IN-Zn 补充相比，HME-Zn 补充显著增加了肝脏中的 SOD。与对照组相比，锌补充降低了丙二醛（MDA）的浓度（P<0.05）。与对照组和 IN-Zn 组相比，饲喂 HME-Zn 的肉鸡的淀粉酶（P<0.05）和胰蛋白酶（P<0.05）活性更高。与对照组相比，十二指肠（P<0.05）和空肠（P<0.05）绒毛高度（VH）的锌补充增加。与对照组相比，HME-Zn 处理可提高空肠绒毛高度（VH）和隐窝深度率（VH:CD）（P<0.05）。与对照组相比，HME-Zn 显著增加了回肠的表观可消化粗蛋白（CP）（P<0.05）和氮校正表观可消化能（AIDEn）（P<0.05）。在第 1 阶段和第 2 阶段，与对照组和 IN-Zn 相比，HME-Zn 显著增加了肝脏和胫骨中的锌浓度（P<0.05）。与 IN-Zn 相比，HME-Zn 组的锌排泄量显著降低（P<0.05）。综上所述，日粮中添加 80mg/kg 的 HME-Zn 可提高肉鸡的生长性能、抗氧化活性、胰腺酶活性、肠道绒毛高度和养分消化率，并提高肉鸡的锌生物利用度。
