基于高分辨 LC-MS 平台的脂质组学全局分析。
Global Lipidomics Profiling by a High Resolution LC-MS Platform.
机构信息
Core Facility Mass Spectrometry, Medical University of Graz, Graz, Austria.
出版信息
Methods Mol Biol. 2021;2306:39-51. doi: 10.1007/978-1-0716-1410-5_3.
PMID:33954938
Abstract
Lipidomics is the determination of big lipid assemblies by mass spectrometry. When using chromatography coupled high resolution mass spectrometry, lipids can be identified by exact mass, fragment spectra, and retention time. This protocol covers lipid extraction, LC-MS data acquisition by Orbitrap mass spectrometry and data processing by Lipid Data Analyzer, a custom developed open source software.
摘要
脂质组学是通过质谱法测定大脂质组装体。当使用色谱法结合高分辨率质谱时，可以通过精确质量、碎片谱和保留时间来鉴定脂质。本方案涵盖了脂质提取、通过轨道阱质谱进行 LC-MS 数据采集以及使用 Lipid Data Analyzer 进行数据处理，后者是一种自定义开发的开源软件。
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