Goh Esther Xue Yi, Guan Xue Li
Lee Kong Chian School of Medicine, Nanyang Technological University, Singapore, Singapore.
Methods Mol Biol. 2021;2306:187-213. doi: 10.1007/978-1-0716-1410-5_13.
Lipids play critical roles in developmental processes, and alterations in lipid metabolism are linked to a wide range of human diseases, including neurodegeneration, cancer, metabolic diseases, and microbial infections. Drosophila melanogaster, more commonly known as the fruit fly, is a powerful organism for developmental biology and human disease research. We have previously developed a comprehensive biochemical tool, based on liquid chromatography-mass spectrometry (LC-MS), to probe the dynamics of lipid remodeling during D. melanogaster development. This chapter introduces a step-by-step protocol for extracting and analyzing lipids across all developmental stages (embryo, larvae, pupa, and adult) of D. melanogaster. The targeted semi-quantitative approach offers a comprehensive coverage of more than 400 lipid species spanning the lipid classes, glycerophospholipids, sphingolipids, triacylglycerols, and sterols.
脂质在发育过程中发挥着关键作用，脂质代谢的改变与多种人类疾病相关，包括神经退行性疾病、癌症、代谢性疾病和微生物感染。黑腹果蝇，通常被称为果蝇，是发育生物学和人类疾病研究的有力生物体。我们之前基于液相色谱 - 质谱联用（LC-MS）开发了一种全面的生化工具，以探究黑腹果蝇发育过程中脂质重塑的动态变化。本章介绍了一种逐步方案，用于提取和分析黑腹果蝇所有发育阶段（胚胎、幼虫、蛹和成虫）的脂质。这种靶向半定量方法全面涵盖了400多种脂质种类，包括甘油磷脂、鞘脂、三酰甘油和固醇等脂质类别。
