利用常规 MALDI-TOF 质谱法进行 shotgun 细菌脂 A 分析。
Shotgun Bacterial Lipid A Analysis Using Routine MALDI-TOF Mass Spectrometry.
机构信息
MRC Centre for Molecular Bacteriology and Infection, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London, UK.
出版信息
Methods Mol Biol. 2021;2306:275-283. doi: 10.1007/978-1-0716-1410-5_18.
PMID:33954953
Abstract
Detection of bacterial lipids and particularly the lipid A, the lipid anchor of the lipopolysaccharide, can be very challenging and requires a certain level of expertise. Here, this chapter describes a straightforward and simple method for the analysis of bacterial lipid A. In addition, such approach, lipid fingerprint, has the potential to be applied to other bacteria such as mycobacteria.
摘要
检测细菌脂质，特别是脂多糖的脂质锚，可能极具挑战性，需要一定的专业知识。在这里，本章描述了一种分析细菌脂 A 的简单直接的方法。此外，这种方法，脂质指纹图谱，有可能应用于其他细菌，如分枝杆菌。
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