Department of Agricultural Botany, Faculty of Agriculture, Kafrelsheikh University, Kafr Elsheikh, Egypt.
Department of Biology, College of Science, Taif University, Taif, Saudi Arabia.
Pest Manag Sci. 2021 Oct;77(10):4350-4356. doi: 10.1002/ps.6468. Epub 2021 May 21.
Induced resistance against several plant pathogens was reported using different beneficial plant growth-promoting microorganisms. The potential of five fungal isolates, Trichoderma harzianum GT 3-2, Fusarium equiseti GF 18-3, F. equiseti GF 19-1, Phoma sp. GS 10-1 and Phoma sp. GS 14-1, to stimulate tomato growth and resistance against bacterial speck disease caused by Pseudomonas syringae pathovar (pv.) tomato DC3000 was evaluated.
Based on the results of disease severity and growth promotion experiments, GF 18-3 exhibited the best results among all fungal isolates. Treatment with barley grain inocula (BGI) and culture filtrate (CF) of the isolates promoted tomato growth and suppressed the pathogen in pot trials. Furthermore, expressions of the pathogenesis-related genes (PR-1, β-1,3-glucanase A, β-1,3-glucanase B and LOX) were relatively higher than the control in the leaves of tomato plants treated with both BGI and CF. The transcription levels remained consistently higher than the control plants for 6 days post-inoculation with pathogen.
Taken together, the results indicate that the tested fungal isolates have the potential to promote tomato growth and induce systemic resistance against the bacterial speck disease. Analysis of certain PR gene expression revealed significant activation in both BGI and CF treatments, leading to stimulated resistance against the pathogen. © 2021 Society of Chemical Industry.
使用不同的有益植物生长促进微生物，已经有报道称其可以诱导对几种植物病原体的抗性。本研究评估了 5 种真菌分离物（哈茨木霉 GT3-2、层出镰刀菌 GF18-3、层出镰刀菌 GF19-1、茎点霉 GS10-1 和茎点霉 GS14-1）刺激番茄生长和对由丁香假单胞菌 pv.番茄 DC3000 引起的细菌性斑点病的抗性潜能。
根据疾病严重程度和生长促进实验的结果，所有真菌分离物中 GF18-3 表现出最佳结果。用大麦粒接种物（BGI）和分离物的培养液滤液（CF）处理在盆栽试验中促进了番茄生长并抑制了病原体。此外，与对照相比，用 BGI 和 CF 处理的番茄叶片中与发病相关的基因（PR-1、β-1,3-葡聚糖酶 A、β-1,3-葡聚糖酶 B 和 LOX）的表达相对较高。在接种病原体后 6 天内，转录水平一直高于对照植物。
综上所述，结果表明，测试的真菌分离物具有促进番茄生长和诱导对细菌性斑点病系统抗性的潜力。对某些 PR 基因表达的分析表明，BGI 和 CF 处理均显著激活，从而刺激了对病原体的抗性。 © 2021 英国化学学会。
