Agricultural Botany Department, Faculty of Agriculture, Kafrelsheikh University, Kafrelsheikh, 33516, Egypt.
Pesticides Chemistry and Toxicology Department, Faculty of Agriculture, Kafrelsheikh University, Kafresheikh, 33516, Egypt.
Environ Sci Pollut Res Int. 2020 Jun;27(16):19049-19057. doi: 10.1007/s11356-018-3806-0. Epub 2018 Nov 27.
This study was conducted to evaluate the ability of zinc oxide nanoparticles (ZONPs) with unique properties to protect tomato against the bacterial speck pathogen, caused by Pseudomonas syringae pv. tomato DC3000 (Pst). Protection of tomato against bacterial speck using ZONPS was evaluated by its direct antibacterial activity and its ability for inducing resistance in tomato plants. The results revealed that ZONPs showed significant direct antibacterial activity against Pseudomonas syringae pv. tomato under laboratory conditions. Moreover, tomato plants treated with ZONPs showed a significant reduction in disease severity and bacterial proliferation relative to non-treated plants. Furthermore, tomato plants treated with ZONPs showed higher self-defense enzyme activity relative to untreated plants. The regulatory and defense genes, LePR-1a and Lipoxygenase (LOX), involved in the salicylic acid (SA) and (JA) signaling pathways, respectively, were highly expressed in tomato plants treated with ZONPs compared to untreated plants. Growth characters of tomato plants treated with ZONPs were significantly enhanced relative to untreated plants. The control of bacterial speck pathogen of tomato using ZONPs through its direct antibacterial and by developing of systemic resistance in treated tomatoes against the pathogen is considered the first report.
本研究旨在评估具有独特性质的氧化锌纳米粒子（ZONPs）保护番茄免受细菌性斑点病原体侵害的能力，该病原体由丁香假单胞菌 pv. 番茄 DC3000（Pst）引起。通过 ZONPs 的直接抗菌活性及其在番茄植物中诱导抗性的能力来评估其对细菌性斑点的保护作用。结果表明，ZONPs 在实验室条件下对丁香假单胞菌 pv. 番茄表现出显著的直接抗菌活性。此外，与未处理的植物相比，用 ZONPs 处理的番茄植物的严重度和细菌增殖显著降低。此外，与未处理的植物相比，用 ZONPs 处理的番茄植物的自我防御酶活性更高。参与水杨酸（SA）和（JA）信号通路的防御基因 LePR-1a 和脂氧合酶（LOX）在 ZONPs 处理的番茄植物中高度表达，而在未处理的植物中则不表达。与未处理的植物相比，用 ZONPs 处理的番茄植物的生长特性显著增强。通过 ZONPs 的直接抗菌作用和在处理的番茄中诱导系统性抗性来控制番茄细菌性斑点病原体被认为是首次报道。
Zotero 插件