Korniienko Nataliia, Kharina Alla, Zrelovs Nikita, Jindřichová Barbora, Moravec Tomaš, Budzanivska Iryna, Burketová Lenka, Kalachova Tetiana
Institute of Experimental Botany of the Czech Academy of Sciences, Prague, Czechia.
Educational and Scientific Center (ESC) "Institute of Biology and Medicine", Taras Shevchenko National University of Kyiv, Kyiv, Ukraine.
Front Microbiol. 2022 Apr 25;13:853593. doi: 10.3389/fmicb.2022.853593. eCollection 2022.
is a bacterial pathogen that causes yield losses in various economically important plant species. At the same time, pv. () is one of the best-studied bacterial phytopathogens and a popular model organism. In this study, we report on the isolation of two phages from the market-bought pepper fruit showing symptoms of bacterial speck. These phages were named Eir4 and Eisa9 and characterized using traditional microbiological methods and whole-genome sequencing followed by various bioinformatics approaches. Both of the isolated phages were capable only of the lytic life cycle and were efficient against several pathovars from and genera. With the combination of transmission electron microscopy (TEM) virion morphology inspection and comparative genomics analyses, both of the phages were classified as members of the family with different degrees of novelty within the known phage diversity. Eir4, but not Eisa9, phage application significantly decreased the propagation of in the leaf tissues of plants. The biological properties of Eir4 phage allow us to propose it as a potential biocontrol agent for use in the prevention of -associated bacterioses and also as a model organism for the future research of mechanisms of phage-host interactions in different plant systems.
是一种细菌病原体，会导致多种具有重要经济价值的植物物种减产。同时，pv. （）是研究最深入的细菌性植物病原体之一，也是一种常用的模式生物。在本研究中，我们报告了从市售的出现细菌性斑点症状的辣椒果实中分离出两种噬菌体。这些噬菌体被命名为Eir4和Eisa9，并使用传统微生物学方法和全基因组测序以及各种生物信息学方法进行了表征。分离出的两种噬菌体都仅具有裂解生命周期，并且对来自和属的几种致病型有效。通过透射电子显微镜（TEM）病毒粒子形态检查和比较基因组学分析相结合，这两种噬菌体都被归类为家族成员，在已知的噬菌体多样性中具有不同程度的新颖性。Eir4噬菌体（而非Eisa9噬菌体）的应用显著降低了植物叶片组织中的繁殖。Eir4噬菌体的生物学特性使我们能够将其作为一种潜在的生物防治剂，用于预防相关的细菌病害，也可作为未来研究不同植物系统中噬菌体-宿主相互作用机制的模式生物。
