Department of Prosthodontics and Materials Science, University of Leipzig, Leipzig, Germany.
Private Dental Practice, Bitburg, Germany.
Clin Exp Dent Res. 2021 Oct;7(5):829-832. doi: 10.1002/cre2.432. Epub 2021 May 6.
This study aimed to evaluate the possible ability of dental impression tray adhesives to serve as a transmission medium for bacteria and fungi when reusable adhesive applicators are utilized.
Ten flasks with tray adhesive were monitored over a period of 12 weeks during clinical use for contamination with bacteria or fungi. Adhesive fluid samples were cultivated on eight different culture media. All grown colonies were identified by using mass spectrometry (MALDI-TOF). Isolates without reliable identification were either identified by Rapid ID 32 API-STREP V3.0 or by sequencing the 16S rRNA genes.
After 4 weeks, bacterial growth was detected on chocolate blood agar plates in five different samples. The bacterial species were identified as Staphylococcus warnerii, Staphylococcus epidermidis, Staphylococcus pasteuri, Ralstonia insidiosa, and Alloiococcus otitidis. After 8 weeks Streptococcus oralis grew on a blood agar plate. In all samples, no fungi were identified.
The disinfectant component of the tested tray adhesive seems to be effective. However, some bacteria survived in the flask for a clinically relevant time, which might result in a potential transmission to a new host.
本研究旨在评估当重复使用黏合剂涂抹器时，牙科印模托盘黏合剂是否有能力作为细菌和真菌的传播媒介。
在临床使用期间，对 10 个装有托盘黏合剂的烧瓶进行了为期 12 周的监测，以观察其是否受到细菌或真菌的污染。将黏合剂液体样本接种在 8 种不同的培养基上进行培养。使用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF）对所有生长的菌落进行鉴定。无法可靠鉴定的分离物通过 Rapid ID 32 API-STREP V3.0 或 16S rRNA 基因测序进行鉴定。
4 周后，在 5 个不同样本的巧克力血琼脂平板上检测到细菌生长。鉴定出的细菌种类为 Warnerella saturnus、表皮葡萄球菌、巴氏葡萄球菌、嗜水气单胞菌和耳炎交链菌。8 周后，血琼脂平板上生长出了口腔链球菌。所有样本中均未鉴定出真菌。
经测试的托盘黏合剂的消毒剂成分似乎有效。然而，一些细菌在烧瓶中存活了相当长的时间，这可能导致潜在的传播给新宿主。
