Natural Product Chemistry Section, CSIR-North East Institute of Science and Technology. Branch Itanagar, Arunachal Pradesh 791110, India.
Anal Methods. 2021 May 7;13(17):2055-2065. doi: 10.1039/d1ay00355k. Epub 2021 Apr 15.
Hydrogen peroxide (HO) has tremendous applications in industry, medicine and in our day-to-day lives. It is toxic to human health upon exposure at a high concentration. Therefore, a green and cost-effective sensing technique is greatly needed for the sensitive naked eye detection of peroxide. This study is mainly focused on the synthesis of Au nanoparticles (AuNPs) using an aqueous extract of Elsholtzia blanda, a flower that is widely available in the North Eastern part of India, the characterization of which was carried out using different analytical techniques. The bioactive molecule (epigallocatechin gallate) present in the aqueous extract was identified, isolated and confirmed through high-performance liquid chromatography-photodiode array, high-resolution mass spectrometry and nuclear magnetic resonance spectroscopy analysis which could be responsible for the reduction of Au ions. By approaching this greener route, the synthesized nanomaterial was further used as a colorimetric probe for the detection of HO and the degradation of AuNPs was observed. The limit of detection was found to be 0.7435 μM in the present work. The degradation of the AuNPs was found to be linearly dependent on peroxide concentration. Along with these results, kinetic studies were carried out by considering different effects to monitor the sensing speed of the AuNPs. The plausible mechanism of the work was supported by density functional theory study.
过氧化氢（HO）在工业、医学和日常生活中有巨大的应用。它在高浓度暴露时对人体健康有毒。因此，非常需要一种绿色且具有成本效益的传感技术，以实现对过氧化物的敏感肉眼检测。本研究主要集中于使用印度东北部广泛存在的一种花——夏枯草（Elsholtzia blanda）的水提物合成金纳米颗粒（AuNPs），使用不同的分析技术对其进行了表征。通过高效液相色谱-光电二极管阵列、高分辨率质谱和核磁共振波谱分析，确定、分离并证实了水提物中存在的生物活性分子（表没食子儿茶素没食子酸酯），该分子可能负责还原金离子。通过采用这种更环保的方法，合成的纳米材料进一步被用作检测 HO 的比色探针，并观察到 AuNPs 的降解。在本工作中，检测限为 0.7435 μM。AuNPs 的降解被发现与过氧化物浓度呈线性相关。除了这些结果外，还通过考虑不同的影响进行了动力学研究，以监测 AuNPs 的传感速度。该工作的合理机制得到了密度泛函理论研究的支持。
