Wang Hao, Liu Zhang, Xiao Jing, Li Chunxiang, Wang Jinglun, Xiao Xiaojuan, Huang Haowen, Shrestha Binita, Tang Liang, Deng Keqin, Zhou Hu
Key Laboratory of Theoretical Organic Chemistry and Function Molecule, Ministry of Education; Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers; Hunan Provincial Key Laboratory of Advanced Materials for New Energy Storage and Conversion; Function Film Engineering Research Center of Hunan Province, Hunan University of Science and Technology, Xiangtan 411201, P. R. China.
School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan 411201, P. R. China.
Anal Chem. 2021 May 18;93(19):7292-7299. doi: 10.1021/acs.analchem.1c00830. Epub 2021 May 6.
Herein, we proposed an innovative visual quantitative sensing strategy based on thiol-ene click chemistry and the capillary action principle. A triethoxyvinylsilane (VTEO)- or mercaptopropylsilatrane (MPS)-modified interface was prepared for analyte recognition. Target analyte molecules containing thiol groups or C═C double bonds are coupled to the VTEO- or MPS-modified inner surface of the glass capillary tube via a thiol-ene click reaction, respectively. Then, the molecular recognition events were transformed into the wettability change of the inner wall of the glass capillary. The concentration of the target molecules was quantified by reading the height change of the water column in the capillary tube. As a proof of concept, this strategy was successfully used to build visual quantitative sensors for detecting glutathione and cholesterol. In addition, this strategy showed a good anti-interference ability to complex biological fluids and realized sensitive glutathione (GSH) and cholesterol detection in real human blood samples.
在此，我们基于硫醇-烯点击化学和毛细作用原理提出了一种创新的视觉定量传感策略。制备了三乙氧基乙烯基硅烷（VTEO）或巯基丙基硅氮烷（MPS）修饰的界面用于分析物识别。含有硫醇基团或碳碳双键的目标分析物分子分别通过硫醇-烯点击反应与玻璃毛细管的VTEO或MPS修饰的内表面偶联。然后，将分子识别事件转化为玻璃毛细管内壁的润湿性变化。通过读取毛细管中水柱的高度变化来定量目标分子的浓度。作为概念验证，该策略成功用于构建检测谷胱甘肽和胆固醇的视觉定量传感器。此外，该策略对复杂生物流体表现出良好的抗干扰能力，并实现了在实际人血样本中灵敏检测谷胱甘肽（GSH）和胆固醇。
