比较从陈旧血样采集物中提取 DNA 的不同方法。
Comparison of DNA extraction methods for samples from old blood collections.
机构信息
Centre for Molecular Medicine & Biobanking, University of Malta, MSD 2080, Malta.
Department of Applied Biomedical Science, Faculty of Health Sciences, University of Malta, MSD 2080, Malta.
出版信息
Biotechniques. 2021 May;70(5):243-250. doi: 10.2144/btn-2020-0113. Epub 2021 May 6.
In this study, DNA was extracted from whole blood which had been collected and stored at -20°C for 5-18 years, with the aim of determining the most suitable commercial DNA extraction kit for this purpose. DNA from nine cord blood samples collected in 1999, 2001 and 2012, with low blood volumes (<1 ml), and a partly dried adult blood sample collected in 2003, having a large blood volume (6 ml) was extracted using four different DNA extraction kits: Quick-DNA Miniprep Plus kit, DNeasy Blood & Tissue kit, MagAttract HMW DNA kit and QIAamp Blood Maxi kit. We concluded that high-quality DNA can be extracted from whole blood sample collections which have been stored for even up to 18 years in a biobank at -20°C.
在这项研究中，从在-20°C 下储存了 5-18 年的全血中提取了 DNA，目的是确定最适合用于此目的的商业 DNA 提取试剂盒。从 1999 年、2001 年和 2012 年收集的 9 份脐带血样本中提取了 DNA，这些样本的血量较少（<1ml），以及 2003 年采集的部分干燥的成人血样本，血量较大（6ml），使用了四种不同的 DNA 提取试剂盒：Quick-DNA Miniprep Plus 试剂盒、DNaeasy Blood & Tissue 试剂盒、MagAttract HMW DNA 试剂盒和 QIAamp Blood Maxi 试剂盒。我们得出结论，即使在-20°C 的生物库中储存长达 18 年，也可以从全血样本中提取高质量的 DNA。
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