GSPA Research Laboratory (Management of Animal Health and Productions), Institute of Veterinary Sciences, University Frères Mentouri Constantine-1, Constantine, Algeria.
Institute of Veterinary and Agronomic Sciences, University Hadj Lakhdar Batna-1, Batna, Algeria.
J Infect Dev Ctries. 2021 Apr 30;15(4):523-529. doi: 10.3855/jidc.13986.
Here, we aim to determine the prevalence of Salmonella contamination of poultry meat from butcheries of the province of Skikda and to investigate antibiotic resistance. Salmonella spp. isolates were screened from 70 samples, including chicken breasts (n = 40 samples) and chicken thighs (n = 30 samples) collected from 14 butcheries. All suspected Salmonella colonies from selective media were confirmed by MALDI-TOF MS and serotyped. The susceptibility profile to 16 antibiotics was studied. According to the antibiotic susceptibility results, resistance genes were investigated by standard PCR targeting various genes such as blaSHV, blaTEM, aac3, aac6-Ibcr, aad, qnrA and qnrB. Of the 14 butcheries studied, samples from eight butcheries were contaminated with Salmonella (57.14%). 19 Salmonella strains were isolated, including five serotypes with a predominance of Kentucky serotype (n = 9), Enteridis (n = 3), followed by Heidelberg (n = 3), Virchow (n = 3), and Manhattan (n= 1). All isolates were resistant to Rifampicin (100%; n = 19), and to other antibiotics such as Ciprofloxacin (47.36%), Amoxicillin-clavulanic acid (47.36%; n = 9), Amoxicillin, (47.36%; n = 9), Ticarcillin-clavulanic acid (47.36%; n = 9), and Gentamycin (47.36%; n = 9). All isolates showing multidrug resistance (47.36%; n = 9) were positive by PCR to the blaTEM-1 β-lactamase gene, from which 8 strains carried the aminoglycoside resistance aad7 gene. However, none was positive for the tested blaSHV, Aac3, Aac6-Ibcr, qnrA, qnrB, ArmA and ArmB genes. Our findings show a worrying rate of Salmonella contamination of poultry meats.
这里，我们旨在确定来自斯基克达省肉铺的家禽肉中沙门氏菌污染的流行情况，并调查抗生素耐药性。从 14 家肉铺采集的 70 个样本（包括鸡胸肉[n=40 个样本]和鸡腿肉[n=30 个样本]）中筛选出了沙门氏菌。从选择性培养基中分离出的所有疑似沙门氏菌均通过 MALDI-TOF MS 进行确认和血清分型。研究了对 16 种抗生素的药敏谱。根据抗生素药敏结果，通过针对 blaSHV、blaTEM、aac3、aac6-Ibcr、aad、qnrA 和 qnrB 等各种基因的标准 PCR 研究了耐药基因。在所研究的 14 家肉铺中，有 8 家肉铺的样本受到沙门氏菌污染（57.14%）。分离出 19 株沙门氏菌，包括以肯塔基血清型（n=9）为主的 5 种血清型，其次是肠沙门氏菌（n=3）、海德堡（n=3）、弗奇奥（n=3）和曼哈顿（n=1）。所有分离株均对利福平（100%；n=19）耐药，对环丙沙星（47.36%）、阿莫西林-克拉维酸（47.36%；n=9）、阿莫西林（47.36%；n=9）、替卡西林-克拉维酸（47.36%；n=9）和庆大霉素（47.36%；n=9）等其他抗生素也耐药。所有显示多药耐药性（47.36%；n=9）的分离株通过 blaTEM-1 内酰胺酶基因的 PCR 检测均为阳性，其中 8 株携带氨基糖苷类耐药 aad7 基因。然而，经测试的 blaSHV、Aac3、Aac6-Ibcr、qnrA、qnrB、ArmA 和 ArmB 基因均未呈阳性。我们的研究结果表明，家禽肉中沙门氏菌污染的比例令人担忧。
