Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, India.
Department of Life Science and Biotechnology, Jadavpur University, Kolkata 700032, India.
Infect Genet Evol. 2021 Aug;92:104892. doi: 10.1016/j.meegid.2021.104892. Epub 2021 May 3.
Novel coronavirus SARS-CoV-2 was recently outbreak worldwide causes severe acute respiratory syndrome along with gastrointestinal symptoms for some infected patients. Information on detail pathogenesis, host immune responses and responsible biological pathways are limited. Therefore, infection specific host gut responses and dietary supplements to neutralize immune inflammation demand extensive research. This study aimed to find differences in global co-expression protein-protein interaction sub-network and enriched biological processes in SARS-CoV and SARS-CoV-2 infected gut enterocytes cell line. Attempts have also been made to predict some dietary supplements to boost human health. The SARS-CoV and SARS-CoV-2 infected differential express proteins were integrated with the human protein interaction network and co-expression subnetworks were constructed. Common hubs of these sub-networks reshape central cellular pathways of metabolic processes, lipid localization, hypoxia response to decrease oxygen level and transport of bio-molecules. The major biological process enriched in the unique hub of SARS-CoV-2 significantly differ from SARS-CoV, related to interferon signaling, regulation of viral process and influenza-A enzymatic pathway. Predicted dietary supplements can improve SARS-CoV-2 infected person''s health by boosting the host immunity/reducing inflammation. To the best of our knowledge this is the first report on co-expression network mediated biological process in human gut enterocytes to predict dietary supplements/compounds.
新型冠状病毒 SARS-CoV-2 最近在全球范围内爆发，导致一些感染患者出现严重的急性呼吸系统综合征和胃肠道症状。关于其详细发病机制、宿主免疫反应和负责的生物学途径的信息有限。因此，需要对感染特异性宿主肠道反应和中和免疫炎症的饮食补充剂进行广泛研究。本研究旨在寻找 SARS-CoV 和 SARS-CoV-2 感染肠上皮细胞系中全球共表达蛋白-蛋白相互作用子网络和富集生物过程的差异。还尝试预测一些饮食补充剂以促进人类健康。将 SARS-CoV 和 SARS-CoV-2 感染的差异表达蛋白与人类蛋白质相互作用网络整合，并构建共表达子网络。这些子网络的共同枢纽重塑了代谢过程、脂质定位、降低氧水平的缺氧反应和生物分子运输的核心细胞途径。SARS-CoV-2 特有枢纽中富集的主要生物学过程与干扰素信号转导、病毒过程调节和流感-A 酶途径显著不同。预测的饮食补充剂可以通过增强宿主免疫力/减少炎症来改善 SARS-CoV-2 感染患者的健康。据我们所知，这是首次在人类肠上皮细胞中报告基于共表达网络的生物学过程，以预测饮食补充剂/化合物。
