A new clitocyboid genus and a new subfamily Cuphophylloideae in the family Hygrophoraceae (Agaricales).

Phylogenetically, the genera , and are treated as basal in the family Hygrophoraceae, despite weak support. However, the exact phylogenetic positions of the three genera have remained unresolved, and taxa related to these genera are poorly known. In this study, a new clitocyboid genus was proposed based on multigenic phylogenetic inference datasets and morphological evidence. The analyses of ITS as well as two combined datasets ITS-nrLSU- and ITS-nrLSU---- supported that (1) formed a well-supported monophyletic clade; and (2) sisters and , along with and also formed a monophyletic lineage, as sister to the rest of the Hygrophoraceae. Meanwhile, two new species, namely and , from southwestern China, were documented and illustrated. These results support the new proposed genus , and that , , and should be retained in the Hygrophoraceae as a new subfamily Cuphophylloideae.