Department of Botany, Box 351330, Johnson 412, University of Washington, Seattle, Washington 98195 USA;
Am J Bot. 2002 Apr;89(4):688-98. doi: 10.3732/ajb.89.4.688.
An investigation of mushroom phylogeny using the largest subunit of RNA polymerase II gene sequences (RPB1) was conducted in comparison with nuclear ribosomal large subunit RNA gene sequences (nLSU) for the same set of taxa in the genus Inocybe (Agaricales, Basidiomycota). The two data sets, though not significantly incongruent, exhibit conflict among the placement of two taxa that exhibit long branches in the nLSU data set. In contrast, RPB1 terminal branch lengths are rather uniform. Bootstrap support is increased for clades in RPB1. Combined data sets increase the degree of confidence for several relationships. Overall, nLSU data do not yield a robust phylogeny when independently assessed by RPB1 sequences. This multigene study indicates that Inocybe is a monophyletic group composed of at least four distinct lineages-subgenus Mallocybe, section Cervicolores, section Rimosae, and subgenus Inocybe sensu Kühner, Kuyper, non Singer. Within subgenus Inocybe, two additional lineages, one composed of species with smooth basidiospores (clade I) and a second characterized by nodulose-spored species (clade II), are recovered by RPB1 and combined data. The nLSU data recover only clade I. The genera Astrosporina and Inocybella cannot be recognized phylogenetically. "Supersections" Cortinatae and Marginatae are not monophyletic groups.
采用最大亚基 RNA 聚合酶 II 基因序列（RPB1）对鹅膏菌属（伞菌目，担子菌门）的同一组分类群进行了蘑菇系统发育研究，与核核糖体大亚基 RNA 基因序列（nLSU）进行了比较。这两个数据集虽然没有明显不一致，但在 nLSU 数据集中表现出两个具有长分支的分类群的位置存在冲突。相比之下，RPB1 末端分支长度相当均匀。RPB1 中的分支得到了更高的自展支持率。联合数据集增加了几个关系的置信度。总体而言，当独立使用 RPB1 序列评估时，nLSU 数据并未产生稳健的系统发育。这项多基因研究表明，鹅膏菌属是一个单系群，由至少四个不同的谱系组成——Mallocybe 亚属、Cervicolores 节、Rimosae 节和 Kühner、Kuyper、非 Singer 意义上的 Inocybe 亚属。在 Inocybe 亚属内，两个额外的谱系，一个由具有光滑担孢子的物种组成（I 类群），另一个由具节孢的物种组成（II 类群），通过 RPB1 和联合数据得到恢复。nLSU 数据仅恢复了 I 类群。Astrosporina 和 Inocybella 属在系统发育上无法识别。“超组”Cortinatae 和 Marginatae 不是单系群。
