Emami Mohammad Hassan, Soltani Shima, Eskandari Nahid, Masjedi Mohsen
Poursina Hakim Digestive Diseases Research Centre, Isfahan University of Medical Sciences, Isahan, Iran.
Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran.
Adv Biomed Res. 2021 Jan 27;10:1. doi: 10.4103/abr.abr_8_20. eCollection 2021.
Celiac disease (CeD) is a chronic inflammatory small intestine disorder caused by an abnormal immune response to an array of the epitopes of the wheat gluten and related proteins of rye and barley in genetically susceptible individuals. Midkine (MK) is an angiogenic cytokine, chemotactic in the direction of polymorphonuclear neutrophils and macrophages, and a T-regulatory cell suppressor. So far, a possible relationship with CeD has not yet been explored. Diagnosis of CeD is based on serologic test in a clinical setting suggestive of CeD and confirmatory histologic examination of the duodenal biopsy. Sometimes, genetic testing of human leukocyte antigen (HLA)-DQ2 and HLA-DQ8 may be needed. The objective of this study was to measure and compare the circulating MK in the celiac patients and healthy individuals.
Twenty newly untreated CeD cases and 20 normal controls were enrolled in this study. The enzyme-linked immunosorbent assay was used to measure the circulating MK in the celiac patients and controls.
There was insignificant difference in the circulating MK between the patients and controls ( > 0.05).
The study results suggest that the MK marker does not have any diagnostic value in CeD activity to be used at the time of diagnosis or during follow-ups.
乳糜泻（CeD）是一种慢性炎症性小肠疾病，由遗传易感个体对小麦麸质以及黑麦和大麦相关蛋白的一系列表位产生异常免疫反应所致。中期因子（MK）是一种血管生成细胞因子，对多形核中性粒细胞和巨噬细胞具有趋化作用，也是一种调节性T细胞抑制因子。到目前为止，尚未探讨其与乳糜泻的可能关系。乳糜泻的诊断基于临床提示乳糜泻时的血清学检测以及十二指肠活检的确诊组织学检查。有时，可能需要进行人类白细胞抗原（HLA）-DQ2和HLA-DQ8的基因检测。本研究的目的是测量和比较乳糜泻患者与健康个体的循环中期因子水平。
本研究纳入了20例新确诊未治疗的乳糜泻病例和20例正常对照。采用酶联免疫吸附测定法测量乳糜泻患者和对照的循环中期因子水平。
患者与对照的循环中期因子水平无显著差异（>0.05）。
研究结果表明，中期因子标志物在乳糜泻诊断或随访时对乳糜泻活动度没有任何诊断价值。
