Dowran Razieh, Malekzadeh Mahyar, Nourollahi Tayyar, Sarkari Bahador, Sarvari Jamal
Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Adv Biomed Res. 2021 Feb 26;10:7. doi: 10.4103/abr.abr_173_20. eCollection 2021.
Protection against hepatitis B virus (HBV) is based on the presence of antibodies against hepatitis B surface antigen (HBsAg). Vaccination of newborns is the most effective means of prevention. This study aimed to evaluate the frequency of anti-HBs antibody (anti-HBsAb), anti-HB core Ab (anti-HBcAb), HBsAg, and HBV DNA among university students in Fars province, Southern Iran.
In this cross-sectional study, 272 students of Shiraz University of Medical Sciences, were enrolled. Venous blood (5 mL) was collected from each participant and centrifuged; the sera were stored at -20°C until use. Anti-HBsAb, Anti-HBcAb, and HBsAg were measured using a commercial enzyme-linked immunosorbent assay kit. HBV DNA load was also measured by a real-time polymerase chain reaction.
The mean age of the participants was 19 ± 1 years. There were 171 (62.9%) females and 101 (37.1%) males. Anti-HBsAb at a protective level (>10 mIU/mL) were detected in the sera of 104 (38.5%) of the cases. Of the anti-HBsAb seropositive participants, 82 were female and 22 were male; the difference between the gender and seropositivity to anti-HBsAb was statistically significant ( = 0.001, odds ratio: 3.3, 95% confidence interval = 1.89-5.79). Anti-HBcAb was detected in only one participant that was negative for both HBsAg and HBV DNA.
Findings of the current study show that more than half of the students do not have a protective level of anti-HBsAb and might be susceptible to HBV infection, indicating the necessity of checking the level of anti-HBsAb as well as a booster dose in high-risk groups.
对乙型肝炎病毒（HBV）的防护基于存在针对乙型肝炎表面抗原（HBsAg）的抗体。新生儿接种疫苗是最有效的预防手段。本研究旨在评估伊朗南部法尔斯省大学生中抗-HBs抗体（抗-HBsAb）、抗-HB核心抗体（抗-HBcAb）、HBsAg和HBV DNA的阳性率。
在这项横断面研究中，招募了设拉子医科大学的272名学生。从每位参与者采集5毫升静脉血并离心；血清储存于-20°C直至使用。使用商用酶联免疫吸附测定试剂盒检测抗-HBsAb、抗-HBcAb和HBsAg。还通过实时聚合酶链反应测量HBV DNA载量。
参与者的平均年龄为19±1岁。有171名（62.9%）女性和101名（37.1%）男性。在104例（38.5%）病例的血清中检测到具有保护水平（>10 mIU/mL）的抗-HBsAb。在抗-HBsAb血清阳性参与者中，82名是女性，22名是男性；性别与抗-HBsAb血清阳性之间的差异具有统计学意义（P = 0.001，比值比：3.3，95%置信区间 = 1.89 - 5.79）。仅在一名HBsAg和HBV DNA均为阴性的参与者中检测到抗-HBcAb。
本研究结果表明，超过一半的学生没有抗-HBsAb的保护水平，可能易感染HBV，这表明有必要检查抗-HBsAb水平以及对高危人群进行加强剂量接种。
