Wirostko E, Johnson L, Wirostko W
Edward S. Harkness Eye Institute, Columbia-Presbyterian Medical Center, New York, N.Y.
J Submicrosc Cytol Pathol. 1988 Apr;20(2):463-70.
Vitritis, the presence of leucocytes in the acellular ocular vitreous, often accompanies ocular vasculitis (V). Non-cultivatable intracellular mollicutes (M), i.e. cell wall deficient bacteria, readily identified by transmission electron microscopy (TEM), are well known plant vascular pathogens. Recently similar intracellular polymorphonuclear leucocytes (PMNL) and lymphocytes (L) parasitising/destroying and mouse ocular and lethal systemic V producing M-like bacteria were reported to be a common cause of human ocular V. In this TEM restudy of 8 human and 3 mouse VM induced chronic ocular V materials severe nuclear and cytoplasmic alterations associated with generalized cytoskeletal nuclear-anchoring 0.005-0.010 micron particles, 0.01-0.05 micron diameter branching filaments and tubules, and trilaminar membrane bound complex internal structure containing 0.1-1.9 micron spherules, all indistinguishable from VM, were observed within 1-3% of the monocytes in all 11 specimens. The results indicate that VM also parasitise and destroy both human and mouse monocytes. VM induced alterations in monocytes, PMNL, and L are compared, and the monocyte nuclear damage/Rifampin beneficial effect relationship in VM induced ocular V is discussed.
玻璃体炎，即无细胞眼玻璃体中存在白细胞，常伴随眼部血管炎（V）。不可培养的细胞内支原体（M），即细胞壁缺陷细菌，通过透射电子显微镜（TEM）很容易识别，是众所周知的植物血管病原体。最近有报道称，类似的寄生/破坏细胞内多形核白细胞（PMNL）和淋巴细胞（L）以及产生M样细菌的小鼠眼部和致死性全身血管炎是人类眼部血管炎的常见原因。在这项对8例人类和3例小鼠VM诱导的慢性眼部血管炎材料的TEM重新研究中，在所有11个标本中1%-3%的单核细胞内观察到与广义细胞骨架核锚定相关的严重核和细胞质改变，包括0.005-0.010微米的颗粒、0.01-0.05微米直径的分支细丝和小管，以及含有0.1-1.9微米小球体的三层膜结合复杂内部结构，所有这些都与VM无法区分。结果表明，VM也寄生并破坏人类和小鼠的单核细胞。比较了VM诱导的单核细胞、PMNL和L的改变，并讨论了VM诱导的眼部血管炎中单核细胞核损伤/利福平有益作用的关系。
