Department of Physiology, Keio University, School of Medicine, Tokyo, Japan.
Methods Mol Biol. 2022;2549:209-217. doi: 10.1007/7651_2021_407.
Amyloid β (Aβ) peptides are the main component of the characteristic insoluble deposits in brain parenchyma and small blood vessels in the patients afflicted with Alzheimer's disease (AD) and cerebral amyloid angiopathy (CAA). These small peptides are attributed to the pathogenesis of both AD and CAA, suggesting an important index for disease stage and progression. In the brain tissue, Aβs are released mainly from neuronal cells into extracellular space. Here, we describe a step-by-step protocol to measure Aβs secreted from human pluripotent stem cell-derived neuronal cells.
淀粉样β（Aβ）肽是阿尔茨海默病（AD）和脑淀粉样血管病（CAA）患者脑实质和小血管中特征性不溶性沉积物的主要成分。这些小肽与 AD 和 CAA 的发病机制有关，提示其是疾病分期和进展的重要指标。在脑组织中，Aβs 主要从神经元细胞释放到细胞外空间。在此，我们描述了一种从人多能干细胞源性神经元细胞中测量分泌的 Aβs 的逐步方案。
