Gnedoĭ S N, Babushkina L M, Levashev V S
Zh Mikrobiol Epidemiol Immunobiol. 1977 Nov(11):111-5.
Deletion plasmide R6Kdelta with the mol wt of 17.2.10(6) dalton isolated from the E. coli chi 925 (R6K) is described. This plasmide expresses no resistance to streptomycin, is replicated in the E. coli K12 under relaxed control and is resistant to the treatment with the eliminating agents. Analysis of plasmide DNA with the aid of electrophoresis in agarose gel demonstrated that R6K delta has one site attacked by restriction endonucleases Eco. RI and Bam HI. These data were confirmed by the determination of the transforming activity of the corresponding DNA restrictors. It is supposed that the isolated plasmide was identical with plasmide RSF1040. A possibility of using R6K delta as a genetic vector for obtaining recombination DNA molecules in vitro is discussed.
描述了从大肠杆菌chi 925（R6K）中分离出的分子量为17.2×10⁶道尔顿的缺失质粒R6Kδ。该质粒对链霉素无抗性，在大肠杆菌K12中在松弛控制下复制，并且对消除剂处理具有抗性。借助琼脂糖凝胶电泳对质粒DNA进行分析表明，R6Kδ有一个被限制性内切酶Eco.RI和Bam HI攻击的位点。相应DNA限制酶的转化活性测定证实了这些数据。推测分离出的质粒与质粒RSF1040相同。讨论了使用R6Kδ作为遗传载体在体外获得重组DNA分子的可能性。
