Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria.
Institute of Pharmacy, Pharmacognosy, Center for Molecular Biosciences (CMBI), University of Innsbruck, Innsbruck, Austria.
J Pharm Biomed Anal. 2021 Jul 15;201:114106. doi: 10.1016/j.jpba.2021.114106. Epub 2021 Apr 27.
Boswellic acids, a class of triterpenes, are the bioactive constituents in Indian frankincense, an herbal drug with pronounced anti-inflammatory activity. In this study their separation and quantification in B. serrata extracts is reported for the first time by using Supercritical Fluid Chromatography. Under optimized conditions, i.e. a Viridis HSS C18 SB column and carbon dioxide, methanol, acetonitrile and ammonium hydroxide as mobile phase, six boswellic acids could be separated in under 6 min. The assay fulfilled all validation criteria with coefficients of determination higher than 0.999, a wide linear range (30-1000 μg/mL), recovery rates from 97.1-103.0 %, excellent precision, and detection limits typical for SFC with UV-detection (≤ 5.5 μg/mL). The method could easily be hyphenated to mass spectrometry, which was helpful to tentatively assign further compounds (mainly derivatives of tirucallic acid) and to increase the assay's sensitivity. Its practical applicability was confirmed by analyzing several commercial products, which mainly contained β-boswellic acid as dominant triterpene, yet in extremely variable amounts ranging from 0.9 to 16.9 %.
乳香酸是一类三萜类化合物，是具有明显抗炎活性的草药药物印度乳香的生物活性成分。本研究首次采用超临界流体色谱法对乳香提取物中的乳香酸进行了分离和定量。在优化条件下，即使用 Viridis HSS C18 SB 柱和二氧化碳、甲醇、乙腈和氨作为流动相，可在 6 分钟内分离出 6 种乳香酸。该测定方法符合所有验证标准，决定系数均高于 0.999，线性范围宽（30-1000μg/mL），回收率为 97.1-103.0%，精密度高，检测限与紫外检测的 SFC 典型值相当（≤5.5μg/mL）。该方法可轻松与质谱联用，有助于对进一步的化合物（主要为 Tirucallic 酸的衍生物）进行初步鉴定，并提高检测的灵敏度。通过分析几种商业产品，证实了该方法的实际适用性，这些产品主要含有β-乳香酸作为主要的三萜类化合物，但含量差异很大，范围从 0.9 到 16.9%。
