Zabihian Mohammad Ali, Hosseini Mehdi, Bahrami Farideh, Iman Maryam, Ghasemi Maedeh, Mohammadi Mohammad Taghi, Bahari Zahra
Student Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
J Complement Integr Med. 2021 May 10;18(4):701-710. doi: 10.1515/jcim-2020-0393.
Resveratrol as a natural polyphenolic agent can alleviate neuropathic pain symptoms. The mechanism of analgesic activity of resveratrol is far from clear. The current study examine whether analgesic activity of resveratrol is mediated by its neuroprotective and anti-oxidant activity in the neuropathic pain. We further examine whether analgesic activity of resveratrol is mediated by β-adrenoceptors in the brain.
Neuropathic pain induced by L spinal nerve ligation (SNL). Male Wistar rats assigned into sham, SNL, SNL + resveratrol (40 μg/5 μL), and SNL + resveratrol + propranolol (a non-selective β-adrenoceptor antagonist, 30 μg/5 μL) groups. Drugs injected intracerebroventricular (ICV) at day SNL surgery and daily for 6 days following SNL. Thermal allodynia and anxiety examined on days of -1, 2, 4, and 6 following SNL. Electrophysiological study performed on day 6 following SNL for evaluation of resveratrol effects on sciatic nerve conduction velocity (NCV). The activity of catalase (Cat) and superoxide dismutase (SOD) enzymes in the brain assessed on days 6 following SNL.
Resveratrol significantly decreased thermal allodynia (and not anxiety) in all experimental days. Additionally, resveratrol significantly increased NCV, and also normalized the disrupted Cat and SOD activities following neuropathic pain. Furthermore, propranolol significantly blocked the analgesic and neuroprotective effects of resveratrol.
It is suggested that the analgesic effects of resveratrol is mediated by its neuroprotective and antioxidant activities in the neuropathic rats. Furthermore, propranolol blocked the analgesic and neuroprotective effects of resveratrol.
白藜芦醇作为一种天然多酚类物质可缓解神经性疼痛症状。白藜芦醇镇痛活性的机制尚不清楚。本研究旨在探讨白藜芦醇的镇痛活性是否通过其在神经性疼痛中的神经保护和抗氧化活性介导。我们还进一步研究白藜芦醇的镇痛活性是否由大脑中的β - 肾上腺素能受体介导。
通过结扎L5脊髓神经（SNL）诱导神经性疼痛。将雄性Wistar大鼠分为假手术组、SNL组、SNL + 白藜芦醇（40μg/5μL）组和SNL + 白藜芦醇 + 普萘洛尔（一种非选择性β - 肾上腺素能受体拮抗剂，30μg/5μL）组。在SNL手术当天经脑室内（ICV）注射药物，并在SNL术后连续6天每日注射。在SNL术后第 -1、2、4和6天检测热痛觉过敏和焦虑情况。在SNL术后第6天进行电生理研究，以评估白藜芦醇对坐骨神经传导速度（NCV）的影响。在SNL术后第6天评估大脑中过氧化氢酶（Cat）和超氧化物歧化酶（SOD）的活性。
在所有实验天数中，白藜芦醇均显著降低热痛觉过敏（而非焦虑）。此外，白藜芦醇显著提高了NCV，并使神经性疼痛后受损的Cat和SOD活性恢复正常。此外，普萘洛尔显著阻断了白藜芦醇的镇痛和神经保护作用。
提示白藜芦醇在神经性大鼠中的镇痛作用是通过其神经保护和抗氧化活性介导的。此外，普萘洛尔阻断了白藜芦醇的镇痛和神经保护作用。
