Students research committee, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Department of Physiology and Medical Physics, Faculty of Medicine, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Arch Razi Inst. 2023 Dec 30;78(6):1852-1860. doi: 10.32592/ARI.2023.78.6.1852. eCollection 2023 Dec.
Neuropathic pain, a chronic pain condition caused by nerve damage either of the peripheral or central nervous system, responds poorly to current drug treatments. The present study aimed to investigate the analgesic and anxiolytic effect of Fe nanoparticles on chronic constriction injury of sciatic nerve (CCI)-induced neuropathic pain in rats. We also assessed the effects of Fe nanoparticles on brain rhythmical oscillation in rats with neuropathic pain. The CCI model was induced by four loose ligations of the left sciatic nerve. Male Wistar rats were divided into four groups: control, sham, CCI, and CCI+Fe nanoparticle (1 mg/kg). The Fe nanoparticle was administered by gavage on the day of CCI surgery (day 0) and daily (once a day) for 21 consecutive days after CCI surgery. Behavioral studies were conducted on days -1, 3, 7, 14, and 21 after CCI. An acetone test and elevated plus maze were performed to evaluate cold allodynia and induced anxiety-like responses, respectively. A field test was conducted to evaluate innate anxiety-like behaviors. In addition, an electrophysiological study was carried out on day 21 after CCI to assess the effects of drugs on brain wave power. Application of Fe significantly reduced cold allodynia in all tested days after CCI, compared to the CCI group. The obtained data demonstrated that Fe nanoparticle gavage caused analgesic and anxiolytic effects on all experimental days after CCI, compared to the CCI group. The CCI surgery significantly disturbed theta, alpha, and beta power in the brain. The application of Fe nanoparticles could not significantly change brain wave power. It is suggested that Fe nanoparticle has analgesic and anxiolytic effects during chronic neuropathic pain in rats. Furthermore, the CCI surgery effectively disturbed brain theta, alpha, and beta power. Nonetheless, the application of Fe nanoparticles could not change deregulated brain oscillation in rats.
神经病理性疼痛是一种由周围或中枢神经系统的神经损伤引起的慢性疼痛病症，对目前的药物治疗反应不佳。本研究旨在探讨 Fe 纳米粒子对慢性坐骨神经结扎（CCI）诱导的大鼠神经病理性疼痛的镇痛和抗焦虑作用。我们还评估了 Fe 纳米粒子对神经病理性疼痛大鼠脑节律性振荡的影响。CCI 模型通过左坐骨神经的 4 个松散结扎诱导。雄性 Wistar 大鼠分为 4 组：对照组、假手术组、CCI 组和 CCI+Fe 纳米粒子（1mg/kg）组。Fe 纳米粒子于 CCI 手术后（第 0 天）灌胃给药，CCI 手术后连续 21 天每天给药（每天一次）。CCI 后第-1、3、7、14 和 21 天进行行为研究。用丙酮试验和高架十字迷宫分别评估冷感觉过敏和诱导的焦虑样反应。进行现场试验以评估先天的焦虑样行为。此外，在 CCI 后第 21 天进行电生理研究，以评估药物对脑电波功率的影响。与 CCI 组相比，Fe 的应用显著减轻了 CCI 后所有测试日的冷感觉过敏。所得数据表明，与 CCI 组相比，Fe 纳米粒子灌胃在 CCI 后所有实验日均产生镇痛和抗焦虑作用。CCI 手术显著干扰了大脑中的θ、α和β波功率。应用 Fe 纳米粒子不能显著改变脑电波功率。这表明 Fe 纳米粒子在大鼠慢性神经病理性疼痛中有镇痛和抗焦虑作用。此外，CCI 手术有效地干扰了大鼠大脑的θ、α和β波功率。然而，应用 Fe 纳米粒子不能改变大鼠大脑中失调的脑振荡。
