Glezerov V Z, Bondarenko V M, Sivochub M I
Zh Mikrobiol Epidemiol Immunobiol. 1977 Sep(9):74-8.
A study was made of 268 cultures isolated from the urine of 263 children suffering from pyelonephritis. Of the total number of different cultures E. coli constituted 79.3 percent; the percentage of the rest varied from 5.2 to 0.4. Examination of 87 urinocultures of E. coli isolated from sick children with the specific immune response showed that the majority of bacterial signs (urease activity, capacity to produce alpha-hemolysin to utilize saccharose and raffinose, to synthesize colicine) failed to correlate with their pyelopathogenicity. The reference to individual serological groups also failed to serve as a sufficient foundation for the separation of these microbes into individual nephropathogenic or pyelopathogenic groups. In experiments with 3H-glucose labeled bacteria there was revealed a marked adhesive capacity in 94 percent of E. coli strains towards the epithelial cells of the RH strain. A positive radioactive label failed to correlate with the presence in E. coli of common pili and with the bacterial agglutinability with the sera K88, K99, and KH-III. The latter pointed to the presence of a factor of unknown nature in the nephropathogenic E. coli strains imparting adhesive properties to bacteria.
对从263例患肾盂肾炎儿童尿液中分离出的268种培养物进行了研究。在所有不同培养物中，大肠杆菌占79.3%；其余细菌的比例从5.2%到0.4%不等。对87例从患病儿童分离出的大肠杆菌尿培养物进行特异性免疫反应检测，结果显示，大多数细菌特征（尿素酶活性、产生α-溶血素的能力、利用蔗糖和棉子糖的能力、合成大肠杆菌素的能力）与其肾盂致病性无关。提及个别血清学组也不足以作为将这些微生物分为个别肾致病性或肾盂致病性组的充分依据。在用3H-葡萄糖标记细菌的实验中，发现94%的大肠杆菌菌株对RH菌株的上皮细胞具有显著的黏附能力。放射性标记呈阳性与大肠杆菌中普通菌毛的存在以及细菌与K88、K99和KH-III血清的凝集性无关。后者表明，肾致病性大肠杆菌菌株中存在一种性质不明的因子，赋予细菌黏附特性。
