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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Masri S W, Ali M, Gubler C J

Department of Biochemistry, Faculty of Science, University of Kuwait, Safat.

Comp Biochem Physiol B. 1988;90(1):167-72. doi: 10.1016/0305-0491(88)90056-9.

Rabbit liver transketolase activity was purified 56-fold using the following steps: ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-25, concentration through an Amicon ultrafiltration cell and rechromatography on DEAE-Sephadex A-25. 2. The enzyme showed an optimum PH for activity at 7.8-8.0. 3. The optimum temperature was around 40 degrees C and the activation energy calculated from the Arrhenius plot was found to be 11.4 kcal/mole. 4. The molecular weight of the enzyme, as determined by gel filtration, was found to be approximately 162,000, while the content of thiamin diphosphate was between 1.8 and 2 mumole per mole protein. 5. Addition of thiamin diphosphate and magnesium chloride did not influence the activity. 6. From the kinetic studies of the enzyme, the Km values for xylulose-5-phosphate, ribose-5-phosphate and fructose-6-phosphate were 3.8 x 10(-5) M, 9.5 x 10(-5) M and 1.1 x 10(-2) M, respectively.

兔肝转酮醇酶活性通过以下步骤纯化了56倍：硫酸铵沉淀、在DEAE-葡聚糖A-25上进行色谱分离、通过Amicon超滤池浓缩以及在DEAE-葡聚糖A-25上再次色谱分离。2. 该酶活性的最适pH为7.8 - 8.0。3. 最适温度约为40℃，根据阿仑尼乌斯曲线计算出的活化能为11.4千卡/摩尔。4. 通过凝胶过滤测定，该酶的分子量约为162,000，而每摩尔蛋白质中硫胺素二磷酸的含量在1.8至2微摩尔之间。5. 添加硫胺素二磷酸和氯化镁不影响活性。6. 根据该酶的动力学研究，5-磷酸木酮糖、5-磷酸核糖和6-磷酸果糖的Km值分别为3.8×10⁻⁵M、9.5×10⁻⁵M和1.1×10⁻²M。

Masri S W, Ali M, Gubler C J

Department of Biochemistry, Faculty of Science, University of Kuwait, Safat.

Comp Biochem Physiol B. 1988;90(1):167-72. doi: 10.1016/0305-0491(88)90056-9.

Rabbit liver transketolase activity was purified 56-fold using the following steps: ammonium sulfate precipitation, chromatography on DEAE-Sephadex A-25, concentration through an Amicon ultrafiltration cell and rechromatography on DEAE-Sephadex A-25. 2. The enzyme showed an optimum PH for activity at 7.8-8.0. 3. The optimum temperature was around 40 degrees C and the activation energy calculated from the Arrhenius plot was found to be 11.4 kcal/mole. 4. The molecular weight of the enzyme, as determined by gel filtration, was found to be approximately 162,000, while the content of thiamin diphosphate was between 1.8 and 2 mumole per mole protein. 5. Addition of thiamin diphosphate and magnesium chloride did not influence the activity. 6. From the kinetic studies of the enzyme, the Km values for xylulose-5-phosphate, ribose-5-phosphate and fructose-6-phosphate were 3.8 x 10(-5) M, 9.5 x 10(-5) M and 1.1 x 10(-2) M, respectively.

兔肝转酮醇酶活性通过以下步骤纯化了56倍：硫酸铵沉淀、在DEAE-葡聚糖A-25上进行色谱分离、通过Amicon超滤池浓缩以及在DEAE-葡聚糖A-25上再次色谱分离。2. 该酶活性的最适pH为7.8 - 8.0。3. 最适温度约为40℃，根据阿仑尼乌斯曲线计算出的活化能为11.4千卡/摩尔。4. 通过凝胶过滤测定，该酶的分子量约为162,000，而每摩尔蛋白质中硫胺素二磷酸的含量在1.8至2微摩尔之间。5. 添加硫胺素二磷酸和氯化镁不影响活性。6. 根据该酶的动力学研究，5-磷酸木酮糖、5-磷酸核糖和6-磷酸果糖的Km值分别为3.8×10⁻⁵M、9.5×10⁻⁵M和1.1×10⁻²M。