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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Kato Y, Iwase H, Hotta K

Department of Biochemistry, School of Medicine, Kitasato University, Sagamihara, Japan.

Comp Biochem Physiol B. 1988;90(1):37-9. doi: 10.1016/0305-0491(88)90034-x.

Ovalbumin was fractionated to six fractions according to their phosphate content by high performance anion exchange chromatography. 2. This method was applied to analyze the phosphate content of ovalbumin subfractions having different carbohydrate chain from each other which were prepared by concanavalin A/Sepharose chromatography from oviduct slices incubated with [2-3H]mannose. 3. Most biosynthetic intermediates bearing a carbohydrate chain of Man8 or 9 GlcNAc2 was not phosphorylated while other fractions bearing differently processed carbohydrate chains such as Man5 or 6 GlcNAc2 or hybrid type carbohydrate chain was phosphorylated at their peptide portion.

通过高效阴离子交换色谱法，根据卵清蛋白的磷酸盐含量将其分离为六个组分。2. 采用该方法分析了通过伴刀豆球蛋白A/琼脂糖凝胶色谱法从用[2-³H]甘露糖孵育的输卵管切片制备的、彼此具有不同碳水化合物链的卵清蛋白亚组分的磷酸盐含量。3. 大多数带有Man8或9 GlcNAc2碳水化合物链的生物合成中间体未被磷酸化，而其他带有不同加工碳水化合物链（如Man5或6 GlcNAc2或杂合型碳水化合物链）的组分在其肽部分被磷酸化。

Kato Y, Iwase H, Hotta K

Department of Biochemistry, School of Medicine, Kitasato University, Sagamihara, Japan.

Comp Biochem Physiol B. 1988;90(1):37-9. doi: 10.1016/0305-0491(88)90034-x.

Ovalbumin was fractionated to six fractions according to their phosphate content by high performance anion exchange chromatography. 2. This method was applied to analyze the phosphate content of ovalbumin subfractions having different carbohydrate chain from each other which were prepared by concanavalin A/Sepharose chromatography from oviduct slices incubated with [2-3H]mannose. 3. Most biosynthetic intermediates bearing a carbohydrate chain of Man8 or 9 GlcNAc2 was not phosphorylated while other fractions bearing differently processed carbohydrate chains such as Man5 or 6 GlcNAc2 or hybrid type carbohydrate chain was phosphorylated at their peptide portion.

通过高效阴离子交换色谱法，根据卵清蛋白的磷酸盐含量将其分离为六个组分。2. 采用该方法分析了通过伴刀豆球蛋白A/琼脂糖凝胶色谱法从用[2-³H]甘露糖孵育的输卵管切片制备的、彼此具有不同碳水化合物链的卵清蛋白亚组分的磷酸盐含量。3. 大多数带有Man8或9 GlcNAc2碳水化合物链的生物合成中间体未被磷酸化，而其他带有不同加工碳水化合物链（如Man5或6 GlcNAc2或杂合型碳水化合物链）的组分在其肽部分被磷酸化。