Kato Y, Iwase H, Hotta K
Anal Biochem. 1984 May 1;138(2):437-41. doi: 10.1016/0003-2697(84)90835-2.
Ovalbumin was fractionated by successive lectin affinity chromatography using concanavalin A/Sepharose and wheat germ agglutinin/Sepharose. Eight glycoprotein fractions, all behaving as ovalbumin on polyacrylamide gel electrophoresis, were obtained. To characterize the carbohydrate chains, the asparaginyl-carbohydrates were prepared from the Pronase digests of the ovalbumin fractions and their dansyl derivatives were analyzed by high-performance liquid chromatography. The elution profile of the dansylated asparaginyl-carbohydrates from each subfraction was compared with that from the unfractionated ovalbumin. The results indicated that the above eight subfractions could be separated from each other according to their carbohydrate chains and that three of the subfractions were homogeneous with respect to their carbohydrate chains.
通过使用伴刀豆球蛋白A/琼脂糖凝胶和麦胚凝集素/琼脂糖凝胶的连续凝集素亲和色谱法对卵清蛋白进行分级分离。获得了八个糖蛋白级分，在聚丙烯酰胺凝胶电泳上均表现为卵清蛋白。为了表征碳水化合物链，从卵清蛋白级分的链霉蛋白酶消化物中制备了天冬酰胺基碳水化合物，并通过高效液相色谱法分析了它们的丹磺酰衍生物。将每个亚级分的丹磺酰化天冬酰胺基碳水化合物的洗脱曲线与未分级的卵清蛋白的洗脱曲线进行比较。结果表明，上述八个亚级分可以根据其碳水化合物链彼此分离，并且其中三个亚级分在碳水化合物链方面是均一的。
