Molecular Biophysics and Integrated Bioimaging Division and the Molecular Foundry, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Environmental Genomics and Systems Biology and Molecular Biophysics and Integrated Bioimaging Divisions, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.
Adv Exp Med Biol. 2022;1371:1-10. doi: 10.1007/5584_2020_615.
Carotenoids are ancient pigment molecules that, when associated with proteins, have a tremendous range of functional properties. Unlike most protein prosthetic groups, there are no recognizable primary structure motifs that predict carotenoid binding, hence the structural details of their amino acid interactions in proteins must be worked out empirically. Here we describe our recent efforts to combine complementary biophysical methods to elucidate the precise details of protein-carotenoid interactions in the Orange Carotenoid Protein and its evolutionary antecedents, the Helical Carotenoid Proteins (HCPs), CTD-like carotenoid proteins (CCPs).
类胡萝卜素是古老的色素分子，与蛋白质结合时具有广泛的功能特性。与大多数蛋白质辅基不同，没有可识别的一级结构模体可以预测类胡萝卜素的结合，因此必须通过实验确定其在蛋白质中的氨基酸相互作用的结构细节。在这里，我们描述了我们最近结合互补生物物理方法来阐明橙色类胡萝卜素蛋白及其进化前体螺旋类胡萝卜素蛋白 (HCPs)、CTD 样类胡萝卜素蛋白 (CCPs) 中蛋白质-类胡萝卜素相互作用的确切细节的努力。
