Jakhmola Shweta, Jha Hem Chandra
Infection Bio-engineering Group, Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, India.
Infection Bio-engineering Group, Discipline of Biosciences and Biomedical Engineering, Indian Institute of Technology Indore, India.
Virology. 2021 Jul;559:182-195. doi: 10.1016/j.virol.2021.04.005. Epub 2021 Apr 21.
Epstein-Barr Virus (EBV) is clinically related to various neurological ailments. The manipulation of neural homeostasis through altered glial cells functions is enigmatic. We investigated EBV mediated nuances in glial cells through direct infection (group-1) or by supplementing them with EBV-infected lymphocytes (PBMCs) supernatant (group-3). Also, the cells were co-cultured with infected PBMCs (group-2). Upon confirmation of infection in U-87 MG through qRT-PCR, the gene expression of crucial molecules was analysed. We reported enhanced expression of IL6 in group-1 and 3 unlike group-2. PBMCs migrated and invaded the matrigel significantly when exposed to group-1 and 3 conditions. Thus, EBV may aid neuroinflammatory reactions through PBMCs infiltration. Also, the exposure of neurons to conditioned supernatant from group-2 caused reduced neuronal healing. Additionally, group-1 milieu contained chemical modulators that induced glial cells death and reduced NF-κB. Conclusively, the three modes of EBV infection can influence glial cells' functions to maneuver the microenvironment distinctly.
爱泼斯坦-巴尔病毒（EBV）在临床上与多种神经系统疾病相关。通过改变神经胶质细胞功能来调控神经内环境稳定的机制尚不清楚。我们通过直接感染（第1组）或用EBV感染的淋巴细胞（外周血单核细胞，PBMCs）上清液处理（第3组）来研究EBV介导的神经胶质细胞细微变化。此外，将细胞与感染的PBMCs共培养（第2组）。通过qRT-PCR确认U-87 MG细胞感染后，分析关键分子的基因表达。我们发现，与第2组不同，第1组和第3组中IL6的表达增强。当暴露于第1组和第3组条件时，PBMCs显著迁移并侵袭基质胶。因此，EBV可能通过PBMCs浸润促进神经炎症反应。此外，将神经元暴露于第2组的条件上清液会导致神经元愈合减少。另外，第1组环境中含有诱导神经胶质细胞死亡并降低NF-κB的化学调节剂。总之，EBV的三种感染模式可不同程度地影响神经胶质细胞功能，进而调控微环境。
