A hydrolytically stable amide polar stationary phase for hydrophilic interaction chromatography.

A novel amide functionalized polar stationary phase has been described by grafting acrylamide polymer coating onto hydrolysed poly (glycidyl methacrylate-divinylbenzene) (GMA-DVB) microsphere. The grafting of acrylamide coating is performed via free radical polymerization of acrylamide with pendant double bonds of hydrolysed GMA-DVB microsphere. The obtained stationary phase (G-pAM) possesses favorable hydrophilicity, as proved by strong retention and good separation ability towards several types of model polar analytes (e.g. 71913 plates/m plate count of sucrose), and excellent hydrolytically stability, as indicated by extremely low bleed level (much superior to commercial ones, 23.7-fold-77.4-fold lower). Negligible baseline drift under gradient elution (water fraction even up to 50%) was observed. It also exhibits good selectivity in the separation of isomers and homologue sugars.