Adam Mickiewicz University, Faculty of Chemistry, Department of Analytical Chemistry, Uniwersytetu Poznańskiego 8, 61-614, Poznań, Poland.
Adam Mickiewicz University, Faculty of Chemistry, Department of Analytical Chemistry, Uniwersytetu Poznańskiego 8, 61-614, Poznań, Poland.
Talanta. 2021 Aug 15;231:122403. doi: 10.1016/j.talanta.2021.122403. Epub 2021 Apr 9.
The paper presents an independent application of two hyphenated techniques, wherein an identical chromatographic system i.e. high performance liquid chromatography (HPLC) was coupled to microwave induced plasma optical emission spectrometry (MIP OES) or inductively coupled plasma optical emission spectrometry (ICP OES). A cation-exchange column and a mobile phase based on pyridine-2,6-dicarboxylic acid (PDCA) were employed to separate Fe(II) and Fe(III) within 300 s. Additionally, two methods of sample preparation were employed. Optimization and validation of both methods were conducted parallel. The applicability was presented with different sample matrix types: post-glacial sediments, archaeological pottery, soils located in the proximity of industry wastes disposal site, river sediments and yerba mate (Ilex paraguariensis). Obtained results were compared in terms of the excitation source (microwave induced or inductively coupled) and supplied gas (nitrogen or argon). The research introduces HPLC-MIP OES for iron speciation analysis and its applicability were critically evaluated with HPLC-ICP OES.
本文独立应用了两种联用技术，其中相同的色谱系统（高效液相色谱法，HPLC）分别与微波诱导等离子体光学发射光谱法（MIP OES）或电感耦合等离子体光学发射光谱法（ICP OES）相连接。采用阳离子交换柱和以吡啶-2,6-二羧酸（PDCA）为基础的流动相，在 300 秒内分离 Fe(II)和 Fe(III)。此外，还采用了两种样品制备方法。对两种方法都进行了优化和验证。并展示了不同样品基质类型的适用性：冰碛沉积物、考古陶器、工业废物处理场附近的土壤、河流沉积物和巴拉圭冬青（Ilex paraguariensis）。从激发源（微波诱导或电感耦合）和供气（氮气或氩气）方面对获得的结果进行了比较。该研究将 HPLC-MIP OES 用于铁形态分析，并与 HPLC-ICP OES 对其适用性进行了批判性评估。
