Department of Analytical Chemistry, Faculty of Chemistry, Adam Mickiewicz University, Uniwersytetu Poznańskiego 8, 61-614 Poznań, Poland.
Faculty of Archaeology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 7, 61-614 Poznań, Poland.
Molecules. 2023 Jun 3;28(11):4539. doi: 10.3390/molecules28114539.
The optimization and application of a new hyphenated procedure for iron ionic speciation, i.e., high performance liquid chromatography (HPLC) with short cation-exchange column (50 mm × 4 mm) coupled to high resolution inductively coupled plasma optical emission spectrometry (ICP hrOES), is presented in this paper. Fe(III) and Fe(II) species were separated on the column with the mobile phase containing pyridine-2,6-dicarboxylic acid (PDCA). The total time of the analysis was approx. 5 min, with a significantly low eluent flow rate (0.5 mL min) compared to the literature. Additionally, a long cation-exchange column (250 mm × 4.0 mm) was used as reference. Depending on the total iron content in the sample, two plasma views were chosen, e.g., an attenuated axial (<2 g kg) and an attenuated radial. The standard addition method was performed for the method's accuracy studies, and the applicability was presented on three types of samples: sediments, soils, and archaeological pottery. This study introduces a fast, efficient, and green method for leachable iron speciation in both geological and pottery samples.
本文介绍了一种新的铁离子形态分析的高效液相色谱-短阳离子交换柱（50mm×4mm）与高分辨率电感耦合等离子体发射光谱（ICP hrOES）联用的方法的优化和应用。Fe（III）和 Fe（II）形态在含有吡啶-2,6-二羧酸（PDCA）的流动相上在柱上分离。与文献相比，分析总时间约为 5 分钟，洗脱液流速显著降低（0.5mL/min）。此外，还使用了长阳离子交换柱（250mm×4.0mm）作为参考。根据样品中总铁的含量，选择两种等离子体视场，例如，轴向衰减（<2gkg）和径向衰减。标准加入法用于方法准确性研究，适用性在三种类型的样品上进行了展示：沉积物、土壤和考古陶器。本研究为地质和陶器样品中可浸提铁形态的快速、高效和绿色方法提供了依据。
