Sugenoya A, Usuda N, Adachi W, Oohashi M, Nagata T, Iida F
Department of Surgery, Shinshu University School of Medicine, Matsumoto, Japan.
Endocrinol Jpn. 1988 Feb;35(1):111-20. doi: 10.1507/endocrj1954.35.111.
Fibronectin (FN) has been considered to be involved in the malignant transformation of cells. It was thus of interest to morphologically study the cell surface distribution of FN in various types of thyroid neoplasms employing immunohistochemical techniques. Immunohistochemical staining using the specific anti-human FN antibody was performed by the avidin-biotin-peroxidase complex procedure. Compared to the staining pattern of normal thyroid tissues (control), differences in staining in malignant thyroid lesions and even in fetal and embryonal adenomas were evident, often on the lateral and basal aspects of the cell membrane. Colloid adenomas, however, showed FN staining on the basement membrane, basically similar to the controls. Moreover, in thyroid carcinoma, different staining patterns relating to each of the histological types were observed. The present investigation demonstrates the heterogeneity of the thyroid neoplasms in terms of FN binding.
纤连蛋白（FN）被认为与细胞的恶性转化有关。因此，利用免疫组织化学技术从形态学上研究FN在各种类型甲状腺肿瘤细胞表面的分布很有意义。采用抗生物素蛋白-生物素-过氧化物酶复合物法，用特异性抗人FN抗体进行免疫组织化学染色。与正常甲状腺组织（对照）的染色模式相比，甲状腺恶性病变甚至胎儿及胚胎性腺瘤的染色差异明显，常在细胞膜的侧面和基底方面。然而，胶样腺瘤在基底膜上显示FN染色，基本与对照相似。此外，在甲状腺癌中，观察到与每种组织学类型相关的不同染色模式。本研究证明了甲状腺肿瘤在FN结合方面的异质性。
