Hespel P, Lijnen P, Fagard R, Van Hoof R, Goossens W, Amery A
Department of Pathophysiology, University of Leuven (K. U.-Leuven), Belgium.
Eur J Appl Physiol Occup Physiol. 1988;57(4):456-61. doi: 10.1007/BF00417993.
The erythrocyte 2,3-diphosphoglycerate concentration (2,3-DPG) and the activity of red cell hexokinase, pyruvate kinase, glucose-6 phosphate dehydrogenase and glutathione reductase were studied in 27 normal volunteers before and after 2 and 4 months of physical endurance training. The 4 months of training increased maximal oxygen uptake and physical working capacity (PWC130) by 16% (p less than 0.001) and 29% (p less than 0.001) respectively. Resting heart rate was decreased (p less than 0.001) by 11 beats.min-1. With 2 months of training the erythrocyte 2,3-DPG concentration increased by 9% (p less than 0.001); with 4 months training the increase was only 4% (p less than 0.05). The training-induced increase in red cell 2,3-DPG was not accompanied by enhanced activity of erythrocyte hexokinase, pyruvate kinase, glucose-6 phosphate dehydrogenase or glutathione reductase. It is concluded that the rise in red cell 2,3-DPG induced by physical endurance training is not due to activation of red cell glycolytic enzymes or the enzymes involved in the pentose-phosphate cycle.
对27名正常志愿者在进行2个月和4个月的耐力训练前后，研究了红细胞2,3 -二磷酸甘油酸浓度（2,3 - DPG）以及红细胞己糖激酶、丙酮酸激酶、葡萄糖 - 6 -磷酸脱氢酶和谷胱甘肽还原酶的活性。4个月的训练使最大摄氧量和体力工作能力（PWC130）分别增加了16%（p<0.001）和29%（p<0.001）。静息心率降低了11次·分钟⁻¹（p<0.001）。经过2个月的训练，红细胞2,3 - DPG浓度增加了9%（p<0.001）；经过4个月的训练，增加幅度仅为4%（p<0.05）。训练引起的红细胞2,3 - DPG增加并未伴随红细胞己糖激酶、丙酮酸激酶、葡萄糖 - 6 -磷酸脱氢酶或谷胱甘肽还原酶活性增强。结论是，耐力训练引起的红细胞2,3 - DPG升高并非由于红细胞糖酵解酶或戊糖磷酸循环中相关酶的激活。
