Fujian Obstetrics and Gynecology Hospital, Fuzhou, Fujian 350011, China; Medical Research Center, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350001, China; Key Laboratory of Technical Evaluation of Fertility Regulation for Non-human Primate, National Health Commission, Fujian Maternity and Child Health Hospital, Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian 350013, China.
Department of Cell Biology and Genetics, The School of Basic Medical Sciences, Fujian Medical University, Fuzhou, Fujian 350122, China.
Res Vet Sci. 2021 Jul;137:127-137. doi: 10.1016/j.rvsc.2021.04.033. Epub 2021 May 4.
Rhesus macaque (Macaca mulatta) is widely applied in animal model construction of infertility, spermatogonia stem cell transplantation and male reproductive diseases. In this review, we describe the seasonal changes of the reproductive system in rhesus macaques, the regular pattern of spermatogenesis and spermatozoa maturation, and the differentiation of spermatogonia and spermatocytes. The duration of the M. mulatta spermatogenesis is approximately 10 days and seminiferous epithelium cycles mainly consist of 12 stages, which provide a suitable model for reproductive studies in non-human primates. Here, we summarize the features of gonadal development and sperm maturation in the rhesus monkeys, which provide important information in the studies of reproductive biology. Rhesus macaque is an excellent animal model in spermatogonia stem cell transplantation. We discuss the applications and progresses of assisted reproductive technologies in sperm liquefaction, semen cryopreservation and spermatogonia stem cell transplantation of rhesus macaques. Besides, we sort out recent proteomic analyses of male reproductive systems and semen samples in rhesus macaques. This review mainly focuses on male reproductive biology and application studies using M. mulatta, which would promote the development of new therapeutic interventions on assisted reproduction and reproductive disease studies in the future.
食蟹猴（Macaca mulatta）广泛应用于不育症、精原干细胞移植和男性生殖疾病的动物模型构建。在这篇综述中，我们描述了食蟹猴生殖系统的季节性变化、精子发生的规律和精子成熟，以及精原细胞和精母细胞的分化。食蟹猴的精子发生大约需要 10 天，生精上皮周期主要由 12 个阶段组成，为非人类灵长类动物的生殖研究提供了合适的模型。在这里，我们总结了食蟹猴性腺发育和精子成熟的特征，为生殖生物学的研究提供了重要信息。食蟹猴是精原干细胞移植的优秀动物模型。我们讨论了辅助生殖技术在食蟹猴精子液化、精液冷冻保存和精原干细胞移植中的应用和进展。此外，我们还梳理了食蟹猴雄性生殖系统和精液样本的最新蛋白质组学分析。本综述主要关注使用 M. mulatta 的雄性生殖生物学和应用研究，这将促进未来辅助生殖和生殖疾病研究中新型治疗干预措施的发展。
