Department of Genetics, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia.
Department of Botany, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Mánesova 23, 04154, Košice, Slovakia.
Phytochemistry. 2021 Jul;187:112742. doi: 10.1016/j.phytochem.2021.112742. Epub 2021 May 6.
In the present study, we performed phytochemical profiling of several under-exploited Hypericum representatives taxonomically belonging to the sections Ascyreia, Androsaemum, Inodora, Hypericum, Coridium, Myriandra, and Adenosepalum. The authenticity of the starting plant material was confirmed using the nuclear ribosomal internal transcribed spacer as a molecular marker, DNA content and chromosome number. Phenolic constituents were analyzed using high-performance liquid chromatography to complement species-specific metabolic profiles. In several Hypericum representatives, the pharmacologically important compounds, including naphthodianthrones; phloroglucinol derivatives; chlorogenic acid; and some classes of flavonoids, particularly the flavonols rutin and hyperoside, flavanol catechin, and flavanones naringenin and naringin, were reported for the first time. Comparative multivariate analysis of chemometric data for seedlings cultured in vitro and acclimated to the outdoor conditions revealed a strong genetically predetermined interspecific variability in phenolic compound content. In addition to hypericins, which are the most abundant chemomarkers for the genus Hypericum, rarely employed phenolic metabolites, including phloroglucinol derivatives, chlorogenic acid, catechin, naringenin, naringin, and kaempferol-3-O-glucoside, were shown to be useful for discriminating between closely related species. Given the increasing interest in natural products of the genus Hypericum, knowledge of the spectrum of phenolic compounds in shoot cultures is a prerequisite for future biotechnological applications. In addition, phytochemical profiling should be considered as an additional part of the integrated plant authentication system, which predominantly relies upon genetic markers.
在本研究中，我们对几个未充分开发的贯叶金丝桃代表种进行了植物化学分析，这些代表种在分类上属于 Ascyreia、Androsaemum、Inodora、Hypericum、Coridium、Myriandra 和 Adenosepalum 组。使用核核糖体内转录间隔区作为分子标记、DNA 含量和染色体数来确认起始植物材料的真实性。使用高效液相色谱法分析酚类成分，以补充物种特异性代谢谱。在几个贯叶金丝桃代表种中，首次报道了一些具有药理重要性的化合物，包括萘并二蒽酮；间苯三酚衍生物；绿原酸；以及一些类黄酮，特别是芸香苷和金丝桃苷、黄烷醇儿茶素、黄烷酮橙皮苷和橙皮苷。对在体外培养并适应户外条件的幼苗进行化学计量数据分析的多元分析表明，在酚类化合物含量方面存在强烈的种间遗传预定变异性。除了贯叶金丝桃素，它是贯叶金丝桃属最丰富的化学标志物外，很少使用的酚类代谢物，包括间苯三酚衍生物、绿原酸、儿茶素、橙皮苷、橙皮苷和山奈酚-3-O-葡萄糖苷，也被证明对区分密切相关的物种有用。鉴于对贯叶金丝桃属天然产物的兴趣日益增加，了解芽培养物中酚类化合物的范围是未来生物技术应用的前提。此外，植物化学分析应被视为综合植物鉴定系统的附加部分，该系统主要依赖于遗传标记。
