State Key Lab for Agrobiotechnology, MOA Key Lab of Soil Microbiology, and College of Biological Sciences, China Agricultural University, Beijing, PR China.
Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, México D. F., México.
J Appl Microbiol. 2021 Nov;131(5):2500-2515. doi: 10.1111/jam.15128. Epub 2021 May 20.
To investigate the effects of three symbiotic Bradyrhizobium strains on peanut growth and on rhizobacterial communities in flowering and harvest stages in an organic farm, also to evaluate the role of plant development in influencing peanut rhizobacterial microbiota and correlations among the inoculants, rhizobacterial communities and plant growth.
Peanut seeds were inoculated with three individual Bradyrhizobium strains, plant growth performance was measured in two developmental stages and rhizobacterial communities were analysed by Illumina sequencing of rpoB gene amplicons from peanut rhizosphere. The three bradyrhizobial inoculants significantly increased the nodule numbers and aboveground fresh weight of peanut plants regardless of the different growth stages, and the pod yields were increased to some extent and significantly positively correlated with Bradyrhizobium abundances in rhizosphere. Principal coordinate analysis indicated that the rhizobacterial communities were strongly influenced by the inoculation and peanut developmental stages. The bradyrhizobia inoculation increased relative abundances of potentially beneficial bacteria in peanut rhizosphere, and also altered rhizobacterial co-occurrence association networks and important network hub taxa. Similarly, plant development also significantly influenced the structure, composition and co-occurrence association networks of rhizobacterial communities.
Bradyrhizobial inoculants increased peanut growth and yields, they and plant development affected the assembly of peanut rhizobacterial communities.
Rhizobial inoculants improved the host plant performance that might also be associated with the dynamic changes in rhizobacterial community except enhancing the biological nitrogen fixation and helps to profoundly understand the mechanism how rhizobia inoculants improve plant growth and yields.
研究 3 种共生慢生根瘤菌菌株对有机农场花生生长和开花期及收获期根际细菌群落的影响，评估植物发育对影响花生根际细菌微生物群落的作用以及接种物、根际细菌群落和植物生长之间的相关性。
将花生种子分别接种到 3 种单独的慢生根瘤菌菌株中，在两个发育阶段测量植物生长性能，并通过 Illumina 测序对花生根际 rpoB 基因扩增子分析根际细菌群落。无论生长阶段如何，这 3 种慢生固氮菌接种剂都显著增加了根瘤的数量和地上部鲜重，并且在一定程度上增加了荚果产量，与根际中的慢生固氮菌丰度呈显著正相关。主坐标分析表明，根际细菌群落受接种和花生发育阶段的强烈影响。根瘤菌接种增加了花生根际中潜在有益细菌的相对丰度，同时改变了根际细菌共生关联网络和重要网络枢纽分类群。同样，植物发育也显著影响了根际细菌群落的结构、组成和共生关联网络。
慢生固氮菌接种剂增加了花生的生长和产量，它们和植物发育影响了花生根际细菌群落的组装。
根瘤菌接种剂改善了宿主植物的性能，这可能除了增强生物固氮作用外，还与根际细菌群落的动态变化有关，有助于深入了解根瘤菌接种剂如何改善植物生长和产量的机制。
