Department of Biotechnology, Middle East Technical University, Ankara, 06800, Turkey.
Department of Engineering Sciences, Middle East Technical University, Ankara, 06800, Turkey.
Carbohydr Polym. 2021 Aug 1;265:118066. doi: 10.1016/j.carbpol.2021.118066. Epub 2021 Apr 12.
This study aims to develop a novel intraperitoneal two- or three-layered patch with immunomodulatory property for treatment of hernia, regeneration of abdominal wall and prevention of intraperitoneal adhesions. Polypropylene (PP) mesh, middle layer, was intended to provide mechanical support whereas pullulan (PUL) hydrogel coating layer was designed to prevent intraperitoneal adhesions. Fibroin/chitosan oligosaccharide lactate (F/COS) layer electrospun on one side of pullulan was chosen for immunomodulation and abdominal wall regeneration. Physical and mechanical properties and regenerative capacity of intraperitoneal patches were determined. Immunomodulatory property of electrospun layer and whole patch was studied by determining nitric oxide amount produced by RAW 264.7 macrophages. 25 % (w/v) PUL hydrogel and F/COS with 90:10 (w/w) ratio yielded optimal results. Here, we report that fabricated intraperitoneal patches successfully prevented cell adhesion on one side and increased cell viability and proliferation on other side, along with immunomodulation, in vitro.
本研究旨在开发一种具有免疫调节特性的新型腹膜内双层或三层补片，用于治疗疝、腹壁再生和预防腹腔粘连。聚丙稀（PP）网片作为中间层，提供机械支撑，而普鲁兰（PUL）水凝胶涂层设计用于防止腹腔粘连。壳聚糖寡糖乳酸（F/COS）纤维层通过静电纺丝的方式置于 PUL 水凝胶的一侧，以达到免疫调节和腹壁再生的目的。对腹膜内补片的物理和机械性能及再生能力进行了测定。通过测定 RAW 264.7 巨噬细胞产生的一氧化氮量来研究电纺层和整个补片的免疫调节特性。25%（w/v）PUL 水凝胶和 F/COS 以 90:10（w/w）的比例达到了最佳效果。本研究报告称，所制备的腹膜内补片在体外成功地防止了一侧的细胞黏附，同时增加了另一侧的细胞活力和增殖，并具有免疫调节作用。
