Laboratory of Biochemistry and Biomedical Materials, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Department of Spinal Surgery, The Affiliated Hospital of Qingdao University, Jiangsu Road 16, Qingdao 266003, PR China.
Carbohydr Polym. 2022 Sep 1;291:119577. doi: 10.1016/j.carbpol.2022.119577. Epub 2022 May 7.
Herein, hydroxypropyl chitosan azide (AZ-HPCTS) was synthesized and prepared as a hydrogel coating applied to a polypropylene mesh (PPM) through UV irradiation. This study confirmed the hypothesis that hydrogels with porous three-dimensional network structures exhibited excellent biocompatibility and biodegradability and adhered well to PPM. During the 180-day follow-up period, the AZ-HPCTS-coated PPM (AH-PPM) promoted wound healing by promoting the secretion of transforming growth factor-beta1 (TGF-beta1) in the acute reaction stage, which was reduced to a lower level at 30 d. The PPM exhibited a lower fibrin lysozyme activity based on the expression of tissue plasminogen activator (tPA) compared with that of AH-PPM (P < 0.05). The intraperitoneal adhesion score of AH-PPM decreased to 2.4 at 180 d in contrast with PPM (P < 0.01), which remained at a high level throughout the study. In conclusion, the AZ-HPCTS hydrogel is a potential coating for hernia patches that deserves further study in the biomaterial field.
在此，通过紫外光照射，将叠氮基羟丙基壳聚糖（AZ-HPCTS）合成并制备为水凝胶涂层，应用于聚丙烯网（PPM）。本研究证实了这样一种假设，即具有多孔三维网络结构的水凝胶表现出极好的生物相容性和生物降解性，并且与 PPM 紧密结合。在 180 天的随访期间，AZ-HPCTS 涂层的 PPM（AH-PPM）通过促进转化生长因子-β1（TGF-β1）在急性反应阶段的分泌促进了伤口愈合，在 30 天减少到较低水平。与 AH-PPM 相比（P<0.05），PPM 基于组织纤溶酶原激活物（tPA）的纤维蛋白溶酶活性较低。与 PPM 相比（P<0.01），AH-PPM 在 180 天的腹腔粘连评分降至 2.4，而在整个研究过程中，粘连评分一直处于较高水平。总之，AZ-HPCTS 水凝胶是疝修补片的一种有潜力的涂层，值得在生物材料领域进一步研究。
