抗性淀粉 3 的消化率受晶体类型、分子量和分子量分布的影响。
Digestibility of resistant starch type 3 is affected by crystal type, molecular weight and molecular weight distribution.
机构信息
Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; Coöperative AVEBE u.a., P.O. Box 15, 9640 AA Veendam, the Netherlands.
出版信息
Carbohydr Polym. 2021 Aug 1;265:118069. doi: 10.1016/j.carbpol.2021.118069. Epub 2021 Apr 16.
Resistant starch type 3 (RS-3) holds great potential as a prebiotic by supporting gut microbiota following intestinal digestion. However the factors influencing the digestibility of RS-3 are largely unknown. This research aims to reveal how crystal type and molecular weight (distribution) of RS-3 influence its resistance. Narrow and polydisperse α-glucans of degree of polymerization (DP) 14-76, either obtained by enzymatic synthesis or debranching amylopectins from different sources, were crystallized in 12 different A- or B-type crystals and in vitro digested. Crystal type had the largest influence on resistance to digestion (A >>> B), followed by molecular weight (Mw) (high DP >> low DP) and Mw distribution (narrow disperse > polydisperse). B-type crystals escaping digestion changed in Mw and Mw distribution compared to that in the original B-type crystals, whereas A-type crystals were unchanged. This indicates that pancreatic α-amylase binds and acts differently to A- or B-type RS-3 crystals.
抗性淀粉类型 3（RS-3）在肠道消化后作为一种支持肠道微生物群的益生元具有很大的潜力。然而，影响 RS-3 可消化性的因素在很大程度上尚不清楚。本研究旨在揭示 RS-3 的晶体类型和分子量（分布）如何影响其抗性。通过酶合成或从不同来源的支链淀粉中解支获得的聚合度（DP）为 14-76 的窄和多分散的α-葡聚糖，在 12 种不同的 A 型或 B 型晶体中结晶，并进行体外消化。晶体类型对消化抗性的影响最大（A ＞＞＞ B），其次是分子量（Mw）（高 DP ＞低 DP）和 Mw 分布（窄分散＞多分散）。与原始 B 型晶体相比，逃避消化的 B 型晶体在 Mw 和 Mw 分布上发生了变化，而 A 型晶体则没有变化。这表明胰腺α-淀粉酶与 A 型或 B 型 RS-3 晶体的结合和作用方式不同。
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