Fugger E F, Bustillo M, Katz L P, Dorfmann A D, Bender S D, Schulman J D
Genetics and IVF Institute, Fairfax, Virginia 22031.
Fertil Steril. 1988 Aug;50(2):273-8. doi: 10.1016/s0015-0282(16)60072-9.
The number of pronucleate (PN) zygotes that cleave, rates of cell division, and pregnancy rates were compared from fresh and frozen sibling PN zygotes. Of 57 patients with randomly selected zygotes frozen at the PN stage, 26 have had 82 PN zygotes thawed with 76 (92.7%) morphologically intact immediately post-thaw. There was no significant difference (P greater than 0.05) in the proportion of fresh (110/123 [89.4%]) and frozen-thawed intact (62/76 [81.6%]) sibling PN zygotes that had cleaved by transfer. The number of fresh versus frozen-thawed sibling PN zygotes that developed into 2-, 3-, 4-, 5-, 6-, 7-, and 8-cell embryos after 24 hours in culture were not significantly different. The numbers of pregnancies per patient cycle for fresh and frozen-thawed sibling PN zygotes were 12/60 (20.0%) and 5/27 (18.5%), respectively (P greater than 0.05). The current cumulative pregnancy rate per retrieval in all cycles with frozen zygotes is 28.3%, considerably higher than observed in single transfers of embryos without cryopreservation (19.8%); predicted cumulative pregnancy rate after eventual transfer of all frozen zygotes is 37.9%. It is concluded that embryo survival and pregnancy rates of cryopreserved PN zygotes are very similar to those from their fresh sibling counterparts and cryopreservation substantially enhances in vitro fertilization pregnancy attainment.
对新鲜和冷冻的同卵原核（PN）受精卵的分裂原核受精卵数量、细胞分裂率和妊娠率进行了比较。在57例在PN阶段随机选择受精卵进行冷冻的患者中，26例解冻了82个PN受精卵，解冻后立即有76个（92.7%）形态完整。新鲜（110/123 [89.4%]）和冻融后完整（62/76 [81.6%]）的同卵PN受精卵在移植后分裂的比例没有显著差异（P大于0.05）。培养24小时后发育成2、3、4、5、6、7和8细胞胚胎的新鲜与冻融后同卵PN受精卵数量没有显著差异。新鲜和冻融后同卵PN受精卵每个患者周期的妊娠数分别为12/60（20.0%）和5/27（18.5%）（P大于0.05）。目前所有冷冻受精卵周期中每次取卵的累积妊娠率为28.3%，远高于未冷冻保存的胚胎单次移植时观察到的妊娠率（19.8%）；最终移植所有冷冻受精卵后的预测累积妊娠率为37.9%。结论是，冷冻保存的PN受精卵的胚胎存活率和妊娠率与其新鲜的同卵对应物非常相似，冷冻保存显著提高了体外受精的妊娠成功率。
