Fugger E F, Bustillo M, Dorfmann A D, Schulman J D
Genetics & IVF Institute, Fairfax, VA 22031.
Hum Reprod. 1991 Jan;6(1):131-5. doi: 10.1093/oxfordjournals.humrep.a137246.
This report describes the results of cryopreserving human preimplantation zygotes and cleaved embryos (2-4 cells) in our in-vitro fertilization programme. Cryopreserved zygotes and cleaved embryos resulted in similar post-thaw survival rates (74.8 versus 70.9%). Pregnancy rates per retrieval cycle (RC) and embryos transferred per pregnancy for frozen-thawed zygotes versus frozen-thawed cleaved embryos were 21.8 versus 11.5% (P less than 0.2) and 12.6 versus 17.5 (P less than 0.2), respectively. Pregnancy rates increased significantly for both fresh (P less than 0.0005) and frozen-thawed (P less than 0.05) embryos as the number of embryos replaced per transfer increased from one to three or more. Frozen-thawed embryos resulted in multiple implantation rates per transfer of 25 compared to 6.4% (P less than 0.1) for fresh embryos when two embryos were replaced. Pregnancy rates were reduced for fresh (P less than 0.05) and frozen-thawed (P less than 0.1) embryos obtained from patient retrieval cycle numbers greater than 3. The method of follicular stimulation during the retrieval cycle did not affect frozen-thawed embryo survival rates. There was no difference in pregnancy rates from frozen-thawed embryos replaced during natural or clomiphene citrate transfer cycles. Patients with cryopreserved embryos had cumulative pregnancy rates of 37.1% (66/178) compared to 23.5% (110/468) (P less than 0.01) for patients with no embryos cryopreserved; cryopreservation of preimplantation embryos is a reliable therapeutic procedure that enhances achievement of pregnancy through in-vitro fertilization.
本报告描述了在我们的体外受精项目中对人类植入前受精卵和分裂胚（2-4细胞）进行冷冻保存的结果。冷冻保存的受精卵和分裂胚解冻后的存活率相似（分别为74.8%和70.9%）。冷冻解冻受精卵与冷冻解冻分裂胚相比，每个取卵周期（RC）的妊娠率以及每次妊娠移植的胚胎数分别为21.8%和11.5%（P<0.2）以及12.6和17.5（P<0.2）。随着每次移植替换的胚胎数从1个增加到3个或更多，新鲜胚胎（P<0.0005）和冷冻解冻胚胎（P<0.05）的妊娠率均显著增加。当移植两个胚胎时，冷冻解冻胚胎每次移植的多胎植入率为25%，而新鲜胚胎为6.4%（P<0.1）。从取卵周期数大于3的患者获得的新鲜胚胎（P<0.05）和冷冻解冻胚胎（P<0.1）的妊娠率降低。取卵周期中的卵泡刺激方法不影响冷冻解冻胚胎的存活率。在自然周期或枸橼酸氯米芬移植周期中移植冷冻解冻胚胎的妊娠率没有差异。有冷冻保存胚胎的患者累积妊娠率为37.1%（66/178），而没有冷冻保存胚胎的患者为23.5%（110/468）（P<0.01）；植入前胚胎的冷冻保存是一种可靠的治疗程序，可通过体外受精提高妊娠成功率。
