Kontsek P, Novák M, Kontseková E
Institute of Virology, Slovak Academy of Sciences, Bratislava.
Folia Biol (Praha). 1988;34(2):99-104.
On the basis of karyotype analysis of the 6 groups of mouse--mouse hybridomas secreting monoclonal antibodies against 5 different antigens we found: (1) The karyotypic variability of clones in all six compared groups of hybridomas was consistent and was influenced neither by the specificity of secreted monoclonal antibodies nor by the character of antigen used for immunization (soluble or particulate). (2) The faster growing hybridomas had a lower mean chromosome number than the slower proliferating clones of the same origin. (3) Karyotypes of subclones isolated from ascites fluid of a mouse inoculated i.p. with clones permanently growing in vitro included a markedly higher mean chromosome number than the karyotypes of originally inoculated hybridoma cells.
基于对6组分泌针对5种不同抗原的单克隆抗体的小鼠-小鼠杂交瘤的核型分析，我们发现：（1）在所有6组比较的杂交瘤中，克隆的核型变异性是一致的，既不受分泌的单克隆抗体的特异性影响，也不受用于免疫的抗原特性（可溶性或颗粒性）影响。（2）生长较快的杂交瘤的平均染色体数低于相同来源的增殖较慢的克隆。（3）从腹腔接种了在体外永久生长的克隆的小鼠腹水中分离出的亚克隆的核型，其平均染色体数明显高于最初接种的杂交瘤细胞的核型。
