Witt S, Ziegler B, Klöting I, Nadrowitz R, Schmidt W, Ziegler M
Zentralinstitut für Diabetes, Gerhardt Katsch Karlsburg.
Allerg Immunol (Leipz). 1987;33(4):259-64.
Hybridoma cells injected intraperitoneally into mice induce formation of ascites tumors producing ascites fluid with high levels of monoclonal antibodies. Several parameters affect the growth of the immunoglobulin-producing tumors in vivo. In the present study the average ascites tumor formation rate of 10 different hybridomas could be increased from 32% (n = 338 mice) to 77% (n = 112 mice) by only one whole body irradiation of paraffin-pretreated-Balb/c mice. Production of monoclonal antibodies was better in males because significantly (p less than 0.01) increased volume of ascites fluid. From the increased tumor formation rate in irradiated mice it is suggested that in non-irradiated recipients the tumor growth rate was lowered by immunological reactions against hybridoma cells provoked by cell surface neoantigens revealed by cell fusion and/or tumor-associated antigens of the myeloma parent cells as well as by altered antigen pattern caused by possible mutations in the myeloma cell line and/or Balb/c/K strain.
将杂交瘤细胞腹腔注射到小鼠体内可诱导腹水肿瘤形成，产生含有高水平单克隆抗体的腹水。有几个参数会影响体内产生免疫球蛋白的肿瘤的生长。在本研究中，仅对经石蜡预处理的Balb/c小鼠进行一次全身照射，10种不同杂交瘤的平均腹水肿瘤形成率就可从32%（n = 338只小鼠）提高到77%（n = 112只小鼠）。雄性小鼠的单克隆抗体产量更高，因为腹水量显著增加（p < 0.01）。从照射小鼠中肿瘤形成率的增加可以推测，在未照射的受体中，肿瘤生长速率因针对杂交瘤细胞的免疫反应而降低，这些免疫反应是由细胞融合所揭示的细胞表面新抗原和/或骨髓瘤亲本细胞的肿瘤相关抗原，以及骨髓瘤细胞系和/或Balb/c/K品系中可能的突变导致的抗原模式改变所引发的。
