Değirmencioğlu Nurcan, Yıldız Elif, Sahan Yasemin, Güldas Metin, Gürbüz Ozan
Department of Food Processing, Vocational High School of Bandirma, University of Bandirma Onyedi Eylul, Balıkesir, Turkey.
Department of Food Engineering, Faculty of Agriculture, University of Bursa Uludag, 16059 Bursa, Turkey.
J Food Sci Technol. 2021 Jun;58(6):2304-2312. doi: 10.1007/s13197-020-04741-7. Epub 2020 Sep 1.
Five different tea varieties (white, green, oolong, black and pu-erh) were infused, drained and used for kombucha production. Antioxidant capacity, total phenolic content and bioaccessibility values were determined. Extractable, hydrolysable and bioaccessible fractions were used for antioxidant capacity and total phenolic content (TPC) and expressed as mole TEAC/mL and mg GAE/100 mL, respectively. Antioxidant capacity and TPC were determined by Folin Ciocalteu's, ABTS and CUPRAC methods. In vitro digestion enzymatic extraction was used to determine bioaccessible fractions. It has found that kombucha fermentation with the tea extracts caused to elevate antioxidant capacity, TPC, and bioaccessibility. The green tea kombucha had the highest antioxidant capacity (4153.23 mole TEAC/mL) and bioaccessibility (50.18%). Therefore, it can be suggested as a good supplement with high nutritional value in kombucha production. In general, TPC content increased significantly. The extractable fractions and bioaccessibility values of the kombucha tea varieties increased due to the fermentation, while the hydrolysable fractions decreased. Antioxidant capacity in the extractable fractions of the green tea kombucha samples was measured to increase by ABTS and CUPRAC methods. Bioaccessibility values of pu-erh tea by ABTS and white tea by CUPRAC were found to increase by the kombucha production.
将五种不同的茶品种(白茶、绿茶、乌龙茶、红茶和普洱茶)进行冲泡、沥干后用于康普茶生产。测定了抗氧化能力、总酚含量和生物可及性值。可提取、可水解和生物可及部分用于测定抗氧化能力和总酚含量(TPC),分别以毫摩尔TEAC/毫升和毫克GAE/100毫升表示。抗氧化能力和TPC通过福林-乔卡泰法、ABTS法和CUPRAC法测定。采用体外消化酶提取法测定生物可及部分。研究发现,用茶提取物进行康普茶发酵可提高抗氧化能力、TPC和生物可及性。绿茶康普茶具有最高的抗氧化能力(4153.23毫摩尔TEAC/毫升)和生物可及性(50.18%)。因此,在康普茶生产中,绿茶可作为一种具有高营养价值的优质补充剂。总体而言,TPC含量显著增加。康普茶品种的可提取部分和生物可及性值因发酵而增加,而可水解部分则减少。通过ABTS法测定绿茶康普茶样品可提取部分的抗氧化能力增加,通过CUPRAC法测定发现普洱茶的生物可及性值和白茶的生物可及性值因康普茶生产而增加。