Rossini Bruno Cesar, de Moraes Mario Luiz Teixeira, Marino Celso Luis
Biotechnology Institute (IBTEC), UNESP-Univ Estadual Paulista, Botucatu, SP CEP 18607-440 Brazil.
Department of Biochemical and Biological Sciences, UNESP-Univ Estadual Paulista, Botucatu, SP CEP 18618-689 Brazil.
Physiol Mol Biol Plants. 2021 Apr;27(4):801-814. doi: 10.1007/s12298-021-00989-1. Epub 2021 Apr 11.
Continuous exploratory use of tree species is threatening the existence of several plants in South America. One of these threatened species is , highly exploited due to the high quality and durability of its wood. The chloroplast (cp) has been used for several evolutionary studies as well traceability of timber origin, based on its gene sequences and simple sequence repeats (SSR) variability. Cp genome organization is usually consisting of a large single copy and a small single copy region separated by two inverted repeats regions. We sequenced the complete cp genome from based on Illumina next-generation sequencing. Our results show that the cp genome is 159,883 bp in size. The 36 SSR identified ranging from mono- to hexanucleotides. Positive selection analysis revealed nine genes related to photosystem, protein synthesis, and DNA replication, and protease are under positive selection. Genome comparison a other Anacardiaceae chloroplast genomes showed great variability in the family. The phylogenetic analysis using complete chloroplast genome sequences of other Anacardiaceae family members showed a close relationship with two other economically important genera, and . These results will help future investigations of timber monitoring and population and evolutionary studies.
The online version contains supplementary material available at 10.1007/s12298-021-00989-1.
对树种的持续探索性利用正威胁着南美洲几种植物的生存。其中一种受威胁的物种是 ，因其木材质量高且耐用而被大量采伐。叶绿体（cp）已被用于多项进化研究以及基于其基因序列和简单序列重复（SSR）变异性的木材来源追溯。叶绿体基因组结构通常由一个大单拷贝和一个小单拷贝区域组成，这两个区域被两个反向重复区域隔开。我们基于Illumina下一代测序技术对 的完整叶绿体基因组进行了测序。我们的结果表明，叶绿体基因组大小为159,883 bp。共鉴定出36个SSR，范围从单核苷酸到六核苷酸。正选择分析揭示了九个与光系统、蛋白质合成、DNA复制以及蛋白酶相关的基因处于正选择状态。与其他漆树科叶绿体基因组的比较显示该科存在很大变异性。使用其他漆树科家族成员的完整叶绿体基因组序列进行的系统发育分析表明，它与另外两个具有重要经济价值的属 和 关系密切。这些结果将有助于未来木材监测以及种群和进化研究。
在线版本包含可在10.1007/s12298-021-00989-1获取的补充材料。
