Gao Yanxia, Hou Linlin, Wang Yibo, Zhang Yan, Zhang Shoutao, Li Yi, Jiang Yanan, Zhu Changju, Sun Tongwen, Duan Guoyu, Yuan Ding
Emergency Department, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Henan Key Laboratory of Bioactive Macromolecules, School of Life Sciences, Zhengzhou, China.
Front Pharmacol. 2021 Apr 20;12:611433. doi: 10.3389/fphar.2021.611433. eCollection 2021.
It is noted that elevated serum amylase levels suggesting pancreatic damage has an association with prognosis in PQ patients. This study aimed to determine whether PQ can cause pancreatic damage. The two conventional models (intragastric infusion (iG) and intraperitoneal injection (iP)) may exhibit different effects on the pancreas depending on whether or not they pass through the digestive tract. In this study, the rats were divided into four groups: the intragastric infusion group (PQ-iG, = 45), intraperitoneal injection group (PQ-iP, = 53), normal control group 1 (NC-iG, = 6) and normal control group 2 (NC-iP, = 6). Pancreatic damage was compared between groups using serum amylase activity assay, hematoxylin and eosin (H&E) staining, TUNEL assay, and transmission electron microscopy (TEM). Serum amylase levels in group PQ-iG were significantly higher than in group PQ-iP ( < 0.05). Examination of the H&E sections showed damage to the pancreas. Both experimental groups were displayed inflammatory infiltration within 9 h of PQ treatment. After 9 h, patchy necrosis was observed in group PQ-iP, when inflammatory infiltration was still the dominant pathology. Necrosis appeared and gradually worsened in group PQ-iG, in which necrosis was the dominant pathology. The TUNEL assay showed significantly higher numbers of apoptotic cells in the pancreas of PQ-groups than in the control NC- groups ( < 0.05). TEM showed expansive endoplasmic reticulum lumens and mitochondria swelling in the pancreas of the PQ-groups. It is concluded that both methods of modeling could cause pancreatic damage and the type and degree of damage would change over time. Note that pancreatic damage in group PQ-iG was more severe than that in group PQ-iP. Therefore, clinical practitioners should pay close attention to pancreatic damage caused by PQ, especially when the route of PQ administration was oral.
值得注意的是，血清淀粉酶水平升高提示胰腺损伤，这与百草枯（PQ）中毒患者的预后相关。本研究旨在确定PQ是否会导致胰腺损伤。两种传统模型（胃内灌注（iG）和腹腔注射（iP））对胰腺的影响可能因是否经过消化道而有所不同。在本研究中，大鼠被分为四组：胃内灌注组（PQ-iG，n = 45）、腹腔注射组（PQ-iP，n = 53）、正常对照组1（NC-iG，n = 6）和正常对照组2（NC-iP，n = 6）。通过血清淀粉酶活性测定、苏木精-伊红（H&E）染色、TUNEL检测和透射电子显微镜（TEM）比较各组之间的胰腺损伤情况。PQ-iG组的血清淀粉酶水平显著高于PQ-iP组（P < 0.05）。H&E切片检查显示胰腺有损伤。两个实验组在PQ处理后9小时内均出现炎症浸润。9小时后，PQ-iP组观察到片状坏死，此时炎症浸润仍是主要病理表现。PQ-iG组出现坏死并逐渐加重，其中坏死是主要病理表现。TUNEL检测显示，PQ组胰腺中的凋亡细胞数量显著高于对照NC组（P < 0.05）。TEM显示PQ组胰腺中内质网腔扩张和线粒体肿胀。结论是，两种建模方法均可导致胰腺损伤，且损伤的类型和程度会随时间变化。请注意，PQ-iG组的胰腺损伤比PQ-iP组更严重。因此，临床医生应密切关注PQ引起的胰腺损伤，尤其是当PQ的给药途径为口服时。
