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分泌型卷曲相关蛋白4促进棕色脂肪细胞分化。

Secreted frizzled-related protein 4 promotes brown adipocyte differentiation.

作者信息

Guan Hua, Zheng Huiyuan, Zhang Jin, Xiang Aoqi, Li Yongxin, Zheng Huadong, Xu Lixian, Liu Enqi, Yu Qi

机构信息

Shaanxi Key Laboratory of Ischemic Cardiovascular Diseases and Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi 710021, P.R. China.

Department of Anesthesiology, The Fourth Military Medical University Stomatological Hospital, Xi'an, Shaanxi 710032, P.R. China.

出版信息

Exp Ther Med. 2021 Jun;21(6):637. doi: 10.3892/etm.2021.10069. Epub 2021 Apr 15.

DOI:10.3892/etm.2021.10069
PMID:33968168
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8097229/
Abstract

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of frizzled proteins. In the present report, the effects of SFRP4 on murine brown adipocyte differentiation were evaluated, which exhibited an intrinsic capacity to differentiate with high efficiency. Brown preadipocytes were isolated from the scapular region of brown adipose tissue, which showed that the overexpression of recombinant active SFRP4 protein at three concentrations (1, 10 and 100 ng/ml) significantly increased the expression of adipocyte differentiation-associated genes (C/EBPα, C/EBPβ, UCP-1, PRDM16, PGC1α and GLUT4) in a dose-dependent manner compared with the control group. Secondly, adiponectin protein expression was significantly inhibited in a dose-independent manner, while leptin was increased in brown adipocytes by incubation with the high concentration (100 ng/ml) of SFRP4. Thirdly, the role of interleukin-1β (IL-1β) was investigated in brown adipocytes and discovered that IL-1β cannot induce SFRP4 mRNA expression in brown adipocytes, similar to human islet cells. These data suggested that SFRP4-treated brown adipocytes represent a valuable model for the study of adipogenesis and indicated that SFRP4 served various functions during brown adipocyte differentiation.

摘要

分泌型卷曲相关蛋白4（SFRP4）是SFRP家族的成员，其含有一个与卷曲蛋白假定的Wnt结合位点同源的富含半胱氨酸的结构域。在本报告中，评估了SFRP4对小鼠棕色脂肪细胞分化的影响，棕色脂肪细胞具有高效分化的内在能力。从棕色脂肪组织的肩胛区域分离出棕色前脂肪细胞，结果显示，与对照组相比，三种浓度（1、10和100 ng/ml）的重组活性SFRP4蛋白的过表达以剂量依赖的方式显著增加了脂肪细胞分化相关基因（C/EBPα、C/EBPβ、UCP-1、PRDM16、PGC1α和GLUT4）的表达。其次，脂联素蛋白表达以剂量非依赖的方式受到显著抑制，而通过与高浓度（100 ng/ml）的SFRP4孵育，棕色脂肪细胞中的瘦素增加。第三，研究了白细胞介素-1β（IL-1β）在棕色脂肪细胞中的作用，发现IL-1β不能诱导棕色脂肪细胞中SFRP4 mRNA的表达，这与人类胰岛细胞类似。这些数据表明，经SFRP4处理的棕色脂肪细胞是研究脂肪生成的有价值模型，并表明SFRP4在棕色脂肪细胞分化过程中发挥了多种功能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/4a1f32de43ed/etm-21-06-10069-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/a88a8818b3f0/etm-21-06-10069-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/a72d221d639a/etm-21-06-10069-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/54035f4e4523/etm-21-06-10069-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/077b05688081/etm-21-06-10069-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/3c4d23b0417a/etm-21-06-10069-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/4a1f32de43ed/etm-21-06-10069-g05.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/a88a8818b3f0/etm-21-06-10069-g00.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/a72d221d639a/etm-21-06-10069-g01.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/54035f4e4523/etm-21-06-10069-g02.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/077b05688081/etm-21-06-10069-g03.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/3c4d23b0417a/etm-21-06-10069-g04.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/83a6/8097229/4a1f32de43ed/etm-21-06-10069-g05.jpg

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