Verspecht Tim, Van Holm Wannes, Boon Nico, Bernaerts Kristel, Daep Carlo A, Masters James G, Zayed Naiera, Quirynen Marc, Teughels Wim
Department of Oral Health Sciences, University of Leuven (KU Leuven), Leuven, Belgium.
Department of Biotechnology, Center for Microbial Ecology and Technology (CMET), Ghent University (UGent), Gent, Belgium.
J Oral Microbiol. 2021 Apr 20;13(1):1910462. doi: 10.1080/20002297.2021.1910462.
: Modulation of the commensal oral microbiota constitutes a promising preventive/therapeutic approach in oral healthcare. The use of prebiotics for maintaining/restoring the health-associated homeostasis of the oral microbiota has become an important research topic. : This study hypothesised that in vitro 14-species oral biofilms can be modulated by (in)direct stimulation of beneficial/commensal bacteria with new potential prebiotic substrates tested at 1 M and 1%, resulting in more host-compatible biofilms with fewer pathogens, decreased virulence and less inflammatory potential. : Established biofilms were repeatedly rinsed with N-acetyl-D-glucosamine, α-D-lactose, D-(+)-trehalose or D-(+)-raffinose at 1 M or 1%. Biofilm composition, metabolic profile, virulence and inflammatory potential were eventually determined. : Repeated rinsing caused a shift towards a more health-associated microbiological composition, an altered metabolic profile, often downregulated virulence gene expression and decreased the inflammatory potential on oral keratinocytes. At 1 M, the substrates had pronounced effects on all biofilm aspects, whereas at 1% they had a pronounced effect on virulence gene expression and a limited effect on inflammatory potential. : Overall, this study identified four new potential prebiotic substrates that exhibit different modulatory effects at two different concentrations that cause in vitro multi-species oral biofilms to become more host-compatible.
口腔共生微生物群的调节是口腔保健中一种很有前景的预防/治疗方法。使用益生元来维持/恢复口腔微生物群与健康相关的稳态已成为一个重要的研究课题。本研究假设，通过用1M和1%浓度测试的新型潜在益生元底物直接或间接刺激有益/共生细菌，可以调节体外14种口腔生物膜，从而产生更多与宿主兼容的生物膜，其中病原体更少，毒力降低，炎症潜力更小。用1M或1%的N-乙酰-D-葡萄糖胺、α-D-乳糖、D-(+)-海藻糖或D-(+)-棉子糖反复冲洗已形成的生物膜。最终确定生物膜的组成、代谢谱、毒力和炎症潜力。反复冲洗导致微生物组成向更健康的方向转变，代谢谱改变，毒力基因表达通常下调，并降低了对口腔角质形成细胞的炎症潜力。在1M浓度下，这些底物对生物膜的各个方面都有显著影响，而在1%浓度下，它们对毒力基因表达有显著影响，对炎症潜力的影响有限。总体而言，本研究确定了四种新型潜在益生元底物，它们在两种不同浓度下表现出不同的调节作用，可使体外多物种口腔生物膜与宿主更兼容。
